1887

Abstract

subsp. is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of subsp. Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (, , , and ), production of plant cell-wall-degrading enzymes (PCWDEs) (, and PCC21_023220), motility ( and ), biofilm formation (, and ), susceptibility to antibacterial plant chemicals () and unknown function (ECA2640). Among the 14 genes identified, , and PCC21_023220 are novel pathogenicity factors of subsp. involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.

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2013-07-01
2020-07-10
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