1887

Abstract

Oxidative stress converts sulfur residues of molecules like biotin and methionine into their oxidized forms. Here we show that the biotin sulfoxide reductase BisC of serovar Typhimurium (. Typhimurium) repairs both oxidized biotin and oxidized methionine. Exposure to HO reduced survival of a Typhimurium Δ mutant. Furthermore, replication of the Δ mutant inside IFN-γ activated macrophages was reduced. tolerance of the mutant to HO was restored by plasmids carrying either or the latter encodes a methioinine sulfoxide reductase. In contrast, the proliferation defect inside IFN-γ activated macrophages was rescued by but not by . Thus growth of the Δ mutant in IFN-γ activated macrophages required repair of oxidized biotin. Both the Δ and a biotin auxotrophic (Δ) mutant were attenuated in mice, suggesting that besides biotin biosynthesis, biotin repair was essential for virulence of . Typhimurium . Attenuation of the Δ mutant was more pronounced in 129 mice that produce a stronger oxidative response. These results show that BisC is essential for full virulence of by contributing to the defence of Typhimurium against host-derived stress, and provides an attractive drug target since it is not present in mammals.

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2013-07-01
2019-10-18
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