%0 Journal Article %A Siwińska, Małgorzata %A Shashkov, Alexander S. %A Kondakova, Anna N. %A Drzewiecka, Dominika %A Zabłotni, Agnieszka %A Arbatsky, Nikolay P. %A Valueva, Olga A. %A Zych, Krystyna %A Sidorczyk, Zygmunt %A Knirel, Yuriy A. %T Structure of the alanopine-containing O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Proteus vulgaris HSC 438 classified into a new Proteus serogroup, O76 %D 2013 %J Microbiology, %V 159 %N Pt_6 %P 1036-1043 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.067231-0 %I Microbiology Society, %X The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of Proteus vulgaris HSC 438, and the following structure was established by chemical methods and one- and two-dimensional 1H and 13C NMR spectroscopy: →3)-β-d-Quip4NAlo-(1→3)-α-d-Galp6Ac-(1→6)-α-d-Glcp-(1→3)-α-l-FucpNAc-(1→3)-β-d-GlcpNAc-(1→, where d-Qui4N stands for 4-amino-4,6-dideoxy-d-glucose and Alo for N-((S)-1-carboxyethyl)-l-alanine (alanopine); only about half of the Gal residues are O-acetylated. This structure is unique among the Proteus O-polysaccharides, and therefore it is proposed to classify P. vulgaris HSC 438 into a new Proteus serogroup, O76. A serological cross-reactivity of HSC 438 O-antiserum and lipopolysaccharides of some other Proteus serogroups was observed and accounted for by shared epitopes on the O-polysaccharides or lipopolysaccharide core regions, including that associated with d-Qui4NAlo. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.067231-0