1887

Abstract

The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of HSC 438, and the following structure was established by chemical methods and one- and two-dimensional H and C NMR spectroscopy: →3)-β--Qui4NAlo-(1→3)-α--Gal6Ac-(1→6)-α--Glc-(1→3)-α--FucNAc-(1→3)-β--GlcNAc-(1→, where -Qui4N stands for 4-amino-4,6-dideoxy--glucose and Alo for -(()-1-carboxyethyl)--alanine (alanopine); only about half of the Gal residues are -acetylated. This structure is unique among the O-polysaccharides, and therefore it is proposed to classify HSC 438 into a new serogroup, O76. A serological cross-reactivity of HSC 438 O-antiserum and lipopolysaccharides of some other serogroups was observed and accounted for by shared epitopes on the O-polysaccharides or lipopolysaccharide core regions, including that associated with -Qui4NAlo.

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2013-06-01
2022-01-29
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