1887

Abstract

The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of HSC 438, and the following structure was established by chemical methods and one- and two-dimensional H and C NMR spectroscopy: →3)-β--Qui4NAlo-(1→3)-α--Gal6Ac-(1→6)-α--Glc-(1→3)-α--FucNAc-(1→3)-β--GlcNAc-(1→, where -Qui4N stands for 4-amino-4,6-dideoxy--glucose and Alo for -(()-1-carboxyethyl)--alanine (alanopine); only about half of the Gal residues are -acetylated. This structure is unique among the O-polysaccharides, and therefore it is proposed to classify HSC 438 into a new serogroup, O76. A serological cross-reactivity of HSC 438 O-antiserum and lipopolysaccharides of some other serogroups was observed and accounted for by shared epitopes on the O-polysaccharides or lipopolysaccharide core regions, including that associated with -Qui4NAlo.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.067231-0
2013-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/159/6/1036.html?itemId=/content/journal/micro/10.1099/mic.0.067231-0&mimeType=html&fmt=ahah

References

  1. Arbatsky N. P., Kondakova A. N., Senchenkova S. N., Siwińska M., Shashkov A. S., Zych K., Knirel Y. A., Sidorczyk Z. ( 2007). Structure of a new ribitol teichoic acid-like O-polysaccharide of a serologically separate Proteus vulgaris strain, TG 276-1, classified into a new Proteus serogroup O53. Carbohydr Res 342:2061–2066 [View Article][PubMed]
    [Google Scholar]
  2. Cedzyński M., Swierzko A. S., Ziółkowski A., Rózalski A., Paramonov N. A., Vinogradov E. V., Knirel Y. A., Kaca W. ( 1998). Structural and immunochemical studies of two cross-reactive Proteus mirabilis O-antigens, O6 and O23, containing β1→3-linked 2-acetamido-2-deoxy-d-glucopyranose residues. Microbiol Immunol 42:7–14[PubMed] [CrossRef]
    [Google Scholar]
  3. Drzewiecka D., Zych K., Sidorczyk Z. ( 2004). Characterization and serological classification of a collection of Proteus penneri clinical strains. Arch Immunol Ther Exp (Warsz) 52:121–128[PubMed]
    [Google Scholar]
  4. Drzewiecka D., Arbatsky N. P., Shashkov A. S., Staczek P., Knirel Y. A., Sidorczyk Z. ( 2008). Structure and serological properties of the O-antigen of two clinical Proteus mirabilis strains classified into a new Proteus O77 serogroup. FEMS Immunol Med Microbiol 54:185–194 [View Article][PubMed]
    [Google Scholar]
  5. Drzewiecka D., Arbatsky N. P., Staczek P., Shashkov A. S., Knirel Y. A., Sidorczyk Z. ( 2010). Structural and serological studies of the O-polysaccharide of strains from a newly created Proteus O78 serogroup prevalent in Polish patients. FEMS Immunol Med Microbiol 58:269–276 [View Article][PubMed]
    [Google Scholar]
  6. Giammanco G. M., Grimont P. A., Grimont F., Lefevre M., Giammanco G., Pignato S. ( 2011). Phylogenetic analysis of the genera Proteus, Morganella and Providencia by comparison of rpoB gene sequences of type and clinical strains suggests the reclassification of Proteus myxofaciens in a new genus, Cosenzaea gen. nov., as Cosenzaea myxofaciens comb. nov. Int J Syst Evol Microbiol 61:1638–1644 [View Article][PubMed]
    [Google Scholar]
  7. Karrer P., Appenzeller R. ( 1942). Die stereoisomeren α,α′-Iminodipropionsäuren. Helv Chim Acta 25:595–599 [View Article]
    [Google Scholar]
  8. Kauffman F. ( 1966). The Bacteriology of Enterobacteriaceae, 3rd edn.333–360 Baltimore, MD: Williams and Wilkins;
    [Google Scholar]
  9. Kawashiro K., Morimoto S., Yoshida H. ( 1984). Mass spectra of butyl esters and N-trifluoroacetyl butyl esters of some iminodicarboxylic acids upon electron impact. Bull Chem Soc Jpn 57:1097–1103 [View Article]
    [Google Scholar]
  10. Knirel Y. A., Perepelov A. V., Kondakova A. N., Senchenkova S. N., Sidorczyk Z., Rozalski A., Kaca W. ( 2011). Structure and serology of O-antigens as the basis for classification of Proteus strains. Innate Immun 17:70–96 [View Article][PubMed]
    [Google Scholar]
  11. Kocharova N. A., Kondakova A. N., Ovchinnikova O. G., Perepelov A. V., Shashkov A. S., Knirel Y. A. ( 2009). N-(1-carboxyethyl)alanine (alanopine), a new non-sugar component of lipopolysaccharides of Providencia and Proteus. . Carbohydr Res 344:2060–2062 [View Article][PubMed]
    [Google Scholar]
  12. Kołodziejska K., Kondakova A. N., Zych K., Senchenkova S. N., Shashkov A. S., Knirel Y. A., Sidorczyk Z. ( 2003). Structure of the O-polysaccharide of a serologically separate strain of Proteus mirabilis, TG 332, from a new proposed Proteus serogroup O50. Carbohydr Res 338:2105–2109 [View Article][PubMed]
    [Google Scholar]
  13. Kołodziejska K., Perepelov A. V., Zabłotni A., Drzewiecka D., Senchenkova S. N., Zych K., Shashkov A. S., Knirel Y. A., Sidorczyk Z. ( 2006). Structure of the glycerol phosphate-containing O-polysaccharides and serological studies of the lipopolysaccharides of Proteus mirabilis CCUG 10704 (OE) and Proteus vulgaris TG 103 classified into a new Proteus serogroup, O54. FEMS Immunol Med Microbiol 47:267–274 [View Article][PubMed]
    [Google Scholar]
  14. Kondakova A. N., Kołodziejska K., Zych K., Senchenkova S. N., Shashkov A. S., Knirel Y. A., Sidorczyk Z. ( 2003). Structure of the N-acetyl-l-rhamnosamine-containing O-polysaccharide of Proteus vulgaris TG 155 from a new Proteus serogroup, O55. Carbohydr Res 338:1999–2004 [View Article][PubMed]
    [Google Scholar]
  15. Kondakova A. N., Linder B., Fudala R., Senchenkova S. N., Moll H., Shashkov A. S., Kaca W., Zähringer U., Knirel Y. A. ( 2004). New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose. Biochemistry (Mosc) 69:1034–1043 [View Article][PubMed]
    [Google Scholar]
  16. Larsson P., Andersson H. E., Norlén B. ( 1978). Serotyping in epidemiological tracing of nosocomially acquired Proteus mirabilis in a geriatric ward. Infection 6:105–110 [View Article][PubMed]
    [Google Scholar]
  17. Leontein K., Lönngren J. ( 1993). Determination of absolute configuration of sugars by gas-liquid chromatography of their acetylated 2-octyl glycosides. Methods Carbohydr Chem 9:87–89
    [Google Scholar]
  18. Lipkind G. M., Shashkov A. S., Knirel Y. A., Vinogradov E. V., Kochetkov N. K. ( 1988). A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-n.m.r. data. Carbohydr Res 175:59–75 [View Article][PubMed]
    [Google Scholar]
  19. Penner J. L., Hennessy J. N. ( 1980). Separate O-grouping schemes for serotyping clinical isolates of Proteus vulgaris and Proteus mirabilis. . J Clin Microbiol 12:304–309[PubMed]
    [Google Scholar]
  20. Perepelov A. V., Babicka D., Senchenkova S. N., Shashkov A. S., Moll H., Rozalski A., Zähringer U., Knirel Y. A. ( 2001). Structure of the O-specific polysaccharide of Proteus vulgaris O4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4-{N-[(R)-3-hydroxybutyryl]-l-alanyl}amino-d-glucose. Carbohydr Res 331:195–202 [View Article][PubMed]
    [Google Scholar]
  21. Perepelov A. V., Rózalski A., Bartodziejska B., Senchenkova S. N., Knirel Y. A. ( 2004). Structure of the O-polysaccharide of Proteus mirabilis O19 and reclassification of certain Proteus strains that were formerly classified in serogroup O19. Arch Immunol Ther Exp (Warsz) 52:188–196[PubMed]
    [Google Scholar]
  22. Perepelov A. V., Zabłotni A., Shashkov A. S., Knirel Y. A., Sidorczyk Z. ( 2006). Structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) containing an amide of d-galacturonic acid with l-alanine. Carbohydr Res 341:1969–1974 [View Article][PubMed]
    [Google Scholar]
  23. Shashkov A. S., Lipkind G. M., Knirel Y. A., Kochetkov N. K. ( 1988). Stereochemical factors determining the effects of glycosylation on the 13C chemical shifts in carbohydrates. Magn Reson Chem 26:735–747 [View Article]
    [Google Scholar]
  24. Sidorczyk Z., Zych K., Toukach F. V., Arbatsky N. P., Zablotni A., Shashkov A. S., Knirel Y. A. ( 2002). Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3. Eur J Biochem 269:1406–1412 [View Article][PubMed]
    [Google Scholar]
  25. Sidorczyk Z., Kondakova A. N., Zych K., Senchenkova S. N., Shashkov A. S., Drzewiecka D., Knirel Y. A. ( 2003). Structure of the O-polysaccharide from Proteus myxofaciens. Classification of the bacterium into a new Proteus O-serogroup. Eur J Biochem 270:3182–3188 [View Article][PubMed]
    [Google Scholar]
  26. Torzewska A., Kondakova A. N., Perepelov A. V., Senchenkova S. N., Shashkov A. S., Rozalski A., Knirel Y. A. ( 2001). Structure of the O-specific polysaccharide of Proteus vulgaris O37 and close serological relatedness of the lipopolysaccharides of P. vulgaris O37 and P. vulgaris O46. FEMS Immunol Med Microbiol 31:227–234 [View Article][PubMed]
    [Google Scholar]
  27. Vinogradov E., Perry M. B. ( 2000). Structural analysis of the core region of lipopolysaccharides from Proteus mirabilis serotypes O6, O48 and O57. Eur J Biochem 267:2439–2447 [View Article][PubMed]
    [Google Scholar]
  28. Vinogradov E. V., Kaca W., Knirel Y. A., Rózalski A., Kochetkov N. K. ( 1989). Structural studies on the fucosamine-containing O-specific polysaccharide of Proteus vulgaris O19. Eur J Biochem 180:95–99 [View Article][PubMed]
    [Google Scholar]
  29. Westphal O., Jann K. ( 1965). Bacterial lipopolysaccharides. Extraction with phenol–water and further applications of the procedure. Methods Carbohydr Chem 5:83–89
    [Google Scholar]
  30. Zabłotni A., Perepelov A. V., Knirel Y. A., Sidorczyk Z. ( 2005). Structure of the O-polysaccharide of Proteus mirabilis OC (CCUG 10702) from a new proposed Proteus serogroup O75. Carbohydr Res 340:1908–1913 [View Article][PubMed]
    [Google Scholar]
  31. Zych K., Kowalczyk M., Knirel Y. A., Sidorczyk Z. ( 2000). New serogroups of the genus Proteus consisting of Proteus penneri strains only. Determination of some LPS epitopes responsible for specificity. Adv Exp Med Biol 485:339–344 [View Article][PubMed]
    [Google Scholar]
  32. Zych K., Perepelov A. V., Siwińska M., Knirel Y. A., Sidorczyk Z. ( 2005). Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups. FEBS J 272:5536–5543 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.067231-0
Loading
/content/journal/micro/10.1099/mic.0.067231-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error