1887

Abstract

The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of HSC 438, and the following structure was established by chemical methods and one- and two-dimensional H and C NMR spectroscopy: →3)-β--Qui4NAlo-(1→3)-α--Gal6Ac-(1→6)-α--Glc-(1→3)-α--FucNAc-(1→3)-β--GlcNAc-(1→, where -Qui4N stands for 4-amino-4,6-dideoxy--glucose and Alo for -(()-1-carboxyethyl)--alanine (alanopine); only about half of the Gal residues are -acetylated. This structure is unique among the O-polysaccharides, and therefore it is proposed to classify HSC 438 into a new serogroup, O76. A serological cross-reactivity of HSC 438 O-antiserum and lipopolysaccharides of some other serogroups was observed and accounted for by shared epitopes on the O-polysaccharides or lipopolysaccharide core regions, including that associated with -Qui4NAlo.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.067231-0
2013-06-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/159/6/1036.html?itemId=/content/journal/micro/10.1099/mic.0.067231-0&mimeType=html&fmt=ahah

References

  1. Arbatsky N. P. , Kondakova A. N. , Senchenkova S. N. , Siwińska M. , Shashkov A. S. , Zych K. , Knirel Y. A. , Sidorczyk Z. . ( 2007; ). Structure of a new ribitol teichoic acid-like O-polysaccharide of a serologically separate Proteus vulgaris strain, TG 276-1, classified into a new Proteus serogroup O53. . Carbohydr Res 342:, 2061–2066. [CrossRef] [PubMed]
    [Google Scholar]
  2. Cedzyński M. , Swierzko A. S. , Ziółkowski A. , Rózalski A. , Paramonov N. A. , Vinogradov E. V. , Knirel Y. A. , Kaca W. . ( 1998; ). Structural and immunochemical studies of two cross-reactive Proteus mirabilis O-antigens, O6 and O23, containing β1→3-linked 2-acetamido-2-deoxy-d-glucopyranose residues. . Microbiol Immunol 42:, 7–14.[PubMed] [CrossRef]
    [Google Scholar]
  3. Drzewiecka D. , Zych K. , Sidorczyk Z. . ( 2004; ). Characterization and serological classification of a collection of Proteus penneri clinical strains. . Arch Immunol Ther Exp (Warsz) 52:, 121–128.[PubMed]
    [Google Scholar]
  4. Drzewiecka D. , Arbatsky N. P. , Shashkov A. S. , Staczek P. , Knirel Y. A. , Sidorczyk Z. . ( 2008; ). Structure and serological properties of the O-antigen of two clinical Proteus mirabilis strains classified into a new Proteus O77 serogroup. . FEMS Immunol Med Microbiol 54:, 185–194. [CrossRef] [PubMed]
    [Google Scholar]
  5. Drzewiecka D. , Arbatsky N. P. , Staczek P. , Shashkov A. S. , Knirel Y. A. , Sidorczyk Z. . ( 2010; ). Structural and serological studies of the O-polysaccharide of strains from a newly created Proteus O78 serogroup prevalent in Polish patients. . FEMS Immunol Med Microbiol 58:, 269–276. [CrossRef] [PubMed]
    [Google Scholar]
  6. Giammanco G. M. , Grimont P. A. , Grimont F. , Lefevre M. , Giammanco G. , Pignato S. . ( 2011; ). Phylogenetic analysis of the genera Proteus, Morganella and Providencia by comparison of rpoB gene sequences of type and clinical strains suggests the reclassification of Proteus myxofaciens in a new genus, Cosenzaea gen. nov., as Cosenzaea myxofaciens comb. nov. . Int J Syst Evol Microbiol 61:, 1638–1644. [CrossRef] [PubMed]
    [Google Scholar]
  7. Karrer P. , Appenzeller R. . ( 1942; ). Die stereoisomeren α,α′-Iminodipropionsäuren. . Helv Chim Acta 25:, 595–599. [CrossRef]
    [Google Scholar]
  8. Kauffman F. . ( 1966; ). The Bacteriology of Enterobacteriaceae, , 3rd edn., pp. 333–360. Baltimore, MD:: Williams and Wilkins;.
    [Google Scholar]
  9. Kawashiro K. , Morimoto S. , Yoshida H. . ( 1984; ). Mass spectra of butyl esters and N-trifluoroacetyl butyl esters of some iminodicarboxylic acids upon electron impact. . Bull Chem Soc Jpn 57:, 1097–1103. [CrossRef]
    [Google Scholar]
  10. Knirel Y. A. , Perepelov A. V. , Kondakova A. N. , Senchenkova S. N. , Sidorczyk Z. , Rozalski A. , Kaca W. . ( 2011; ). Structure and serology of O-antigens as the basis for classification of Proteus strains. . Innate Immun 17:, 70–96. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kocharova N. A. , Kondakova A. N. , Ovchinnikova O. G. , Perepelov A. V. , Shashkov A. S. , Knirel Y. A. . ( 2009; ). N-(1-carboxyethyl)alanine (alanopine), a new non-sugar component of lipopolysaccharides of Providencia and Proteus. . Carbohydr Res 344:, 2060–2062. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kołodziejska K. , Kondakova A. N. , Zych K. , Senchenkova S. N. , Shashkov A. S. , Knirel Y. A. , Sidorczyk Z. . ( 2003; ). Structure of the O-polysaccharide of a serologically separate strain of Proteus mirabilis, TG 332, from a new proposed Proteus serogroup O50. . Carbohydr Res 338:, 2105–2109. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kołodziejska K. , Perepelov A. V. , Zabłotni A. , Drzewiecka D. , Senchenkova S. N. , Zych K. , Shashkov A. S. , Knirel Y. A. , Sidorczyk Z. . ( 2006; ). Structure of the glycerol phosphate-containing O-polysaccharides and serological studies of the lipopolysaccharides of Proteus mirabilis CCUG 10704 (OE) and Proteus vulgaris TG 103 classified into a new Proteus serogroup, O54. . FEMS Immunol Med Microbiol 47:, 267–274. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kondakova A. N. , Kołodziejska K. , Zych K. , Senchenkova S. N. , Shashkov A. S. , Knirel Y. A. , Sidorczyk Z. . ( 2003; ). Structure of the N-acetyl-l-rhamnosamine-containing O-polysaccharide of Proteus vulgaris TG 155 from a new Proteus serogroup, O55. . Carbohydr Res 338:, 1999–2004. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kondakova A. N. , Linder B. , Fudala R. , Senchenkova S. N. , Moll H. , Shashkov A. S. , Kaca W. , Zähringer U. , Knirel Y. A. . ( 2004; ). New structures of the O-specific polysaccharides of Proteus. 4. Polysaccharides containing unusual acidic N-acyl derivatives of 4-amino-4,6-dideoxy-D-glucose. . Biochemistry (Mosc) 69:, 1034–1043. [CrossRef] [PubMed]
    [Google Scholar]
  16. Larsson P. , Andersson H. E. , Norlén B. . ( 1978; ). Serotyping in epidemiological tracing of nosocomially acquired Proteus mirabilis in a geriatric ward. . Infection 6:, 105–110. [CrossRef] [PubMed]
    [Google Scholar]
  17. Leontein K. , Lönngren J. . ( 1993; ). Determination of absolute configuration of sugars by gas-liquid chromatography of their acetylated 2-octyl glycosides. . Methods Carbohydr Chem 9:, 87–89.
    [Google Scholar]
  18. Lipkind G. M. , Shashkov A. S. , Knirel Y. A. , Vinogradov E. V. , Kochetkov N. K. . ( 1988; ). A computer-assisted structural analysis of regular polysaccharides on the basis of 13C-n.m.r. data. . Carbohydr Res 175:, 59–75. [CrossRef] [PubMed]
    [Google Scholar]
  19. Penner J. L. , Hennessy J. N. . ( 1980; ). Separate O-grouping schemes for serotyping clinical isolates of Proteus vulgaris and Proteus mirabilis. . J Clin Microbiol 12:, 304–309.[PubMed]
    [Google Scholar]
  20. Perepelov A. V. , Babicka D. , Senchenkova S. N. , Shashkov A. S. , Moll H. , Rozalski A. , Zähringer U. , Knirel Y. A. . ( 2001; ). Structure of the O-specific polysaccharide of Proteus vulgaris O4 containing a new component of bacterial polysaccharides, 4,6-dideoxy-4-{N-[(R)-3-hydroxybutyryl]-l-alanyl}amino-d-glucose. . Carbohydr Res 331:, 195–202. [CrossRef] [PubMed]
    [Google Scholar]
  21. Perepelov A. V. , Rózalski A. , Bartodziejska B. , Senchenkova S. N. , Knirel Y. A. . ( 2004; ). Structure of the O-polysaccharide of Proteus mirabilis O19 and reclassification of certain Proteus strains that were formerly classified in serogroup O19. . Arch Immunol Ther Exp (Warsz) 52:, 188–196.[PubMed]
    [Google Scholar]
  22. Perepelov A. V. , Zabłotni A. , Shashkov A. S. , Knirel Y. A. , Sidorczyk Z. . ( 2006; ). Structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) containing an amide of d-galacturonic acid with l-alanine. . Carbohydr Res 341:, 1969–1974. [CrossRef] [PubMed]
    [Google Scholar]
  23. Shashkov A. S. , Lipkind G. M. , Knirel Y. A. , Kochetkov N. K. . ( 1988; ). Stereochemical factors determining the effects of glycosylation on the 13C chemical shifts in carbohydrates. . Magn Reson Chem 26:, 735–747. [CrossRef]
    [Google Scholar]
  24. Sidorczyk Z. , Zych K. , Toukach F. V. , Arbatsky N. P. , Zablotni A. , Shashkov A. S. , Knirel Y. A. . ( 2002; ). Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3. . Eur J Biochem 269:, 1406–1412. [CrossRef] [PubMed]
    [Google Scholar]
  25. Sidorczyk Z. , Kondakova A. N. , Zych K. , Senchenkova S. N. , Shashkov A. S. , Drzewiecka D. , Knirel Y. A. . ( 2003; ). Structure of the O-polysaccharide from Proteus myxofaciens. Classification of the bacterium into a new Proteus O-serogroup. . Eur J Biochem 270:, 3182–3188. [CrossRef] [PubMed]
    [Google Scholar]
  26. Torzewska A. , Kondakova A. N. , Perepelov A. V. , Senchenkova S. N. , Shashkov A. S. , Rozalski A. , Knirel Y. A. . ( 2001; ). Structure of the O-specific polysaccharide of Proteus vulgaris O37 and close serological relatedness of the lipopolysaccharides of P. vulgaris O37 and P. vulgaris O46. . FEMS Immunol Med Microbiol 31:, 227–234. [CrossRef] [PubMed]
    [Google Scholar]
  27. Vinogradov E. , Perry M. B. . ( 2000; ). Structural analysis of the core region of lipopolysaccharides from Proteus mirabilis serotypes O6, O48 and O57. . Eur J Biochem 267:, 2439–2447. [CrossRef] [PubMed]
    [Google Scholar]
  28. Vinogradov E. V. , Kaca W. , Knirel Y. A. , Rózalski A. , Kochetkov N. K. . ( 1989; ). Structural studies on the fucosamine-containing O-specific polysaccharide of Proteus vulgaris O19. . Eur J Biochem 180:, 95–99. [CrossRef] [PubMed]
    [Google Scholar]
  29. Westphal O. , Jann K. . ( 1965; ). Bacterial lipopolysaccharides. Extraction with phenol–water and further applications of the procedure. . Methods Carbohydr Chem 5:, 83–89.
    [Google Scholar]
  30. Zabłotni A. , Perepelov A. V. , Knirel Y. A. , Sidorczyk Z. . ( 2005; ). Structure of the O-polysaccharide of Proteus mirabilis OC (CCUG 10702) from a new proposed Proteus serogroup O75. . Carbohydr Res 340:, 1908–1913. [CrossRef] [PubMed]
    [Google Scholar]
  31. Zych K. , Kowalczyk M. , Knirel Y. A. , Sidorczyk Z. . ( 2000; ). New serogroups of the genus Proteus consisting of Proteus penneri strains only. Determination of some LPS epitopes responsible for specificity. . Adv Exp Med Biol 485:, 339–344. [CrossRef] [PubMed]
    [Google Scholar]
  32. Zych K. , Perepelov A. V. , Siwińska M. , Knirel Y. A. , Sidorczyk Z. . ( 2005; ). Structures of the O-polysaccharides and classification of Proteus genomospecies 4, 5 and 6 into respective Proteus serogroups. . FEBS J 272:, 5536–5543. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.067231-0
Loading
/content/journal/micro/10.1099/mic.0.067231-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error