@article{mbs:/content/journal/micro/10.1099/mic.0.066472-0, author = "Bateman, Stacey L. and Stapleton, Ann E. and Stamm, Walter E. and Hooton, Thomas M. and Seed, Patrick C.", title = "The type 1 pili regulator gene fimX and pathogenicity island PAI-X as molecular markers of uropathogenic Escherichia coli", journal= "Microbiology", year = "2013", volume = "159", number = "Pt_8", pages = "1606-1617", doi = "https://doi.org/10.1099/mic.0.066472-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.066472-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Uropathogenic Escherichia coli (UPEC) fall within a larger group of isolates producing extraintestinal disease. UPEC express type 1 pili as a critical virulence determinant mediating adherence to and invasion into urinary tract tissues. Type 1 pili expression is under regulation by a family of site-specific recombinases, including FimX, which is encoded from a genomic island called PAI-X for pathogenicity island of FimX. Using a new multiplex PCR, fimX and the additional PAI-X genes were found to be highly associated with UPEC (144/173 = 83.2 %), and more prevalent in UPEC of lower urinary tract origin (105/120 = 87.5 %) than upper urinary tract origin (39/53 = 74 %; P<0.05) or commensal isolates (28/78 = 36 %; P≤0.0001). The Fim-like recombinase gene fimX is the only family member that has a significant association with UPEC compared to commensal isolates. Our results indicate PAI-X genes, including the type 1 pili regulator gene fimX, are highly prevalent among UPEC isolates and have a strong positive correlation with genomic virulence factors, suggesting a potential role for PAI-X in the extraintestinal pathogenic E. coli lifestyle.", }