1887

Abstract

OmpW is a minor porin whose biological function has not been clearly defined. Evidence obtained in our laboratory indicates that in serovar Typhimurium the expression of OmpW is activated by SoxS upon exposure to paraquat and it is required for resistance. SoxS belongs to the AraC family of transcriptional regulators, like MarA and Rob. Due to their high structural similarity, the genes under their control have been grouped in the // regulon, which presents a DNA-binding consensus sequence denominated the box. In this work, we evaluated the role of the transcription factors MarA, SoxS and Rob of serovar Typhimurium in regulating expression in response to menadione. We determined the transcript and protein levels of OmpW in different genetic backgrounds; in the wild-type and Δ strains was upregulated in response to menadione, while in the Δ and Δ strains the induction was abolished. In a double mutant, transcript levels were lowered after exposure to menadione, and only complementation with both genes restored the positive regulation. Using transcriptional fusions and electrophoretic mobility shift assays with mutant versions of the promoter region we demonstrated that two of the predicted sites were functional. Additionally, we demonstrated that MarA increases the affinity of SoxS for the promoter region. In conclusion, our study shows that is upregulated in response to menadione in a cooperative manner by MarA and SoxS through a direct interaction with the promoter region.

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2013-04-01
2020-01-29
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