1887

Abstract

In a previous study, two proteins identified as hyaluronidases were detected in spent media by MS and found to be in greater quantity in the and mutant strains when compared with the parent and mutant strains of UAMS-1. In the present study, spent media and total RNA were isolated from UAMS-1 and its regulatory mutants and analysed for hyaluronidase activity and steady-state hyaluronidase () RNA message levels. Hyaluronidase activity was observed throughout all time points examined regardless of the regulatory effects of and but activity was always substantially higher in the and mutant strains than in the UAMS-1 parent and mutant strains. Northern analysis did not detect message for either the UAMS-1 parent or the mutant strains at any time point examined, while steady-state message levels were detected throughout growth for the mutant strain, but only at exponential and early post-exponential growth for the mutant strain. An biofilm plate assay, pre-coated with human plasma as a source of hyaluronic acid, demonstrated no significant increase in biofilm for a mutant strain of UAMS-1 defective in hyaluronidase activity when compared with the mutant strain. These data indicate that, while message levels and hyaluronidase activity are elevated in the mutant strains of UAMS-1, the increase in activity did not contribute to the biofilm-negative phenotype observed in the mutant strain of UAMS-1.

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2013-04-01
2019-10-15
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