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Volume 159, Issue Pt_4

Identification and characterization of a cluster of genes involved in biosynthesis and transport of acinetoferrin, a siderophore produced by ATCC 17906 Free

Abstract

ATCC 17906 is known to produce the siderophore acinetoferrin under iron-limiting conditions. Here, we show that an operon consisting of eight consecutive genes, named and , participates in the biosynthesis and transport of acinetoferrin, respectively. Transcription of the operon was found to be iron-regulated by a putative Fur box located in the promoter region of the first gene, . Homology searches suggest that and encode enzyme proteins involved in acinetoferrin biosynthesis and an outer-membrane receptor for ferric acinetoferrin, respectively. Mutants defective in and were unable to produce acinetoferrin or to express the ferric acinetoferrin receptor under iron-limiting conditions. These abilities were rescued by complementation of the mutants with native and genes. Secondary structure analysis predicted that the products of and may be inner-membrane proteins with 12 membrane-spanning helices that belong to the major facilitator superfamily proteins. ActC showed homology to RhtX, which has been characterized as an inner-membrane importer for ferric rhizobactin 1021 structurally similar to acinetoferrin. Compared to the parental ATCC 17906 strain, the mutant displayed about a 35 % reduction in secretion of acinetoferrin, which was restored by complementation with , suggesting that ActD acts as an exporter of the siderophore. Finally, the product was significantly similar to hypothetical proteins in certain bacteria, in which genes encoding ActBCA homologues are arranged in the same order as in . ATCC 17906. However, the function of ActB remains to be clarified.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science, and Technology of Japan (Award 22790140)
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2013-04-01
2024-04-25
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Identification and characterization of a cluster of genes involved in biosynthesis and transport of acinetoferrin, a siderophore produced by Acinetobacter haemolyticus ATCC 17906T
Microbiology 159, 678 (2013); https://doi.org/10.1099/mic.0.065177-0
/content/journal/micro/10.1099/mic.0.065177-0
/content/journal/micro/10.1099/mic.0.065177-0
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