RT Journal Article SR Electronic(1) A1 Malamud, Florencia A1 Homem, Rafael Augusto A1 Conforte, Valeria Paola A1 Yaryura, Pablo Marcelo A1 Castagnaro, Atilio Pedro A1 Marano, María Rosa A1 do Amaral, Alexandre Morais A1 Vojnov, Adrián AlbertoYR 2013 T1 Identification and characterization of biofilm formation-defective mutants of Xanthomonas citri subsp. citri JF Microbiology, VO 159 IS Pt_9 SP 1911 OP 1919 DO https://doi.org/10.1099/mic.0.064709-0 PB Microbiology Society, SN 1465-2080, AB Xanthomonas citri subsp. citri (Xcc) develops a biofilm structure both in vitro and in vivo. Despite all the progress achieved by studies regarding biofilm formation, many of its mechanisms remain poorly understood. This work focuses on the identification of new genes involved in biofilm formation and how they are related to motility, virulence and chemotaxis in Xcc. A Tn5 library of approximately 6000 Xcc (strain 306) mutants was generated and screened to search for biofilm formation defective strains. We identified 23 genes not previously associated with biofilm formation. The analysis of the 23 mutants not only revealed the involvement of new genes in biofilm formation, but also reinforced the importance of exopolysaccharide production, motility and cell surface structures in this process. This collection of biofilm-defective mutants underscores the multifactorial genetic programme underlying the establishment of biofilm in Xcc., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.064709-0