1887

Abstract

The signalling molecule bis-(3′–5′)-cyclic-dimeric guanosine monophosphate (c-di-GMP) is a central regulator of diverse cellular functions, including motility, biofilm formation, cell cycle progression and virulence, in bacteria. Multiple diguanylate cyclase and phosphodiesterase-domain-containing proteins (GGDEF and EAL/HD-GYP, respectively) modulate the levels of the second messenger c-di-GMP to transmit signals and obtain such specific cellular responses. In the genus this c-di-GMP network is poorly studied. In this work, we evaluated the expression of two phenotypes in regulated by c-di-GMP, biofilm formation and motility, under the influence of ectopic expression of proteins with EAL or GGDEF domains that regulates the c-di-GMP level. In agreement with previous reports for other bacteria, we observed that is able to form biofilm and reduce its motility only when GGDEF domain protein is expressed. Moreover we identify a GGDEF domain protein (BB3576) with diguanylate cyclase activity that participates in motility and biofilm regulation in . These results demonstrate for the first time, to our knowledge, the presence of c-di-GMP regulatory signalling in .

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2013-05-01
2022-07-05
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