1887

Abstract

is an opportunistic fungal pathogen which is a growing concern for immunocompromised patients. It is ranked as the second most common cause of candidiasis after . For pathogenic yeasts, intracellular pH (pH) has been implicated in proliferation, dimorphic switching and virulence. We expressed the pH-sensitive green fluorescent protein variant ratiometric pHluorin in the cytosol of to study pH dynamics in living cells. We evaluated the response of pH to the various growth and stress conditions encountered during interaction with the host and during antifungal treatment. maintained a pH higher than that of in all growth conditions. The pH of cells appeared better controlled than the pH in when the cells were exposed to food and fermentation-associated conditions. in turn maintained its pH better when exposed to host-associated conditions.

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2013-04-01
2020-05-28
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