1887

Abstract

, a class II transposable element, was characterized in the genome. The transposase is highly similar to plant -like transposases that belong to the newest class II superfamily known as /. Although shares characteristics with -like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that belongs to a novel family of the / superfamily. Southern blot analyses detected 6–12 copies of in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the hybridization profile after the sexual cycle and detection of transcripts gave further evidence of activity. As an active family in the genome of , elements may contribute to genetic variability in this homothallic fungus. This is the first report of a / transposon in the genome of a phytopathogenic fungus.

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2013-01-01
2022-01-28
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