1887

Abstract

is the pathogen that causes Glässer’s disease, a major illness affecting young pigs. The aim of this work was to investigate the antagonistic activity of antimicrobial substances produced by species against . Among the tested strains, only ATCC 6633 inhibited growth. The antibacterial substance was purified by ammonium sulfate precipitation, gel filtration chromatography on Sephadex G-50 and ion-exchange chromatography on DEAE-cellulose. The purification was about 100-fold with a yield of 0.33 %. The purified substance was resistant up to 80 °C and pH ranging 3–7, but the substance lost its activity when it was treated with proteases. The peptide had a molecular mass of 1083 Da and its sequence was determined by MS as NRWCFAGDD, which showed no homology with other known antimicrobial peptides. The complete inhibition of growth was observed at 20 µg peptide ml after 20 min of exposure. The peptide obtained by chemical synthesis also showed antimicrobial activity on . The identification of antimicrobial substances that can be effective against is very relevant to combat this pathogen that causes important losses in swine production.

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2013-05-01
2019-10-15
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