1887

Abstract

We recently showed that the two-component system (TCS) HrrSA plays a central role in the control of haem homeostasis in the Gram-positive soil bacterium Here, we characterized the function of another TCS of this organism, ChrSA, which exhibits significant sequence similarity to HrrSA, and provide evidence for cross-regulation of the two systems. In this study, ChrSA was shown to be crucial for haem resistance of by activation of the putative haem-detoxifying ABC-transporter HrtBA in the presence of haem. Deletion of either or resulted in a strongly increased sensitivity towards haem. DNA microarray analysis and gel retardation assays with the purified response regulator ChrA revealed that phosphorylated ChrA acts as an activator of in the presence of haem. The haem oxygenase gene, , showed a decreased mRNA level in a deletion mutant but no significant binding of ChrA to the promoter was observed . In contrast, activation from P fused to was almost abolished in an mutant, indicating that HrrSA is the dominant system for haem-dependent activation of in . Remarkably, ChrA was also shown to bind to the promoter and to repress transcription of the paralogous response regulator, whereas itself seemed to be repressed by HrrA. These data suggest a close interplay of HrrSA and ChrSA at the level of transcription and emphasize ChrSA as a second TCS involved in haem-dependent gene regulation in , besides HrrSA.

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2012-12-01
2020-07-10
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