1887

Abstract

possesses a complex cell wall that is unique and essential for interaction of the pathogen with its human host. Emerging evidence suggests that the biosynthesis of complex cell-wall lipids is mediated by serine/threonine protein kinases (STPKs). Herein, we show, using radiolabelling, MS and immunostaining analyses, that targeted deletion of one of the STPKs, attenuates the production of phthiocerol dimycocerosates (PDIMs), a major virulence lipid. Comparative protein expression analysis revealed that proteins in the PDIM biosynthetic pathway are differentially expressed in a deleted strain. Furthermore, we analysed the composition of the major lipoglycans, lipoarabinomannan (LAM) and lipomannan (LM), and found a twofold higher LAM/LM ratio in the mutant strain. Thus, we provide experimental evidence that PknH contributes to the production and synthesis of cell-wall components.

Funding
This study was supported by the:
  • Canadian Institute of Health Research (CIHR) (Award MOP-106622 )
  • Medical Research Council
  • Wellcome Trust
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2013-04-01
2022-09-25
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