1887

Abstract

Extracytoplasmic function (ECF) sigma factors (σ) are known to bring about changes in gene expression to enable bacteria to adapt to different stresses. The Sp245 genome harbours nine genes encoding σ, of which two are adjacent to the genes encoding ChrR-type zinc-binding anti-sigma (ZAS) factors. We describe here the role and regulation of a new pair of , which was found in the genome of Sp7 in addition to the previously described pair (designated ). The pair is also cotranscribed, and their products show protein–protein interaction. The −10 and −35 promoter elements of and were similar but not identical. Unlike the promoter of , the promoter was neither autoregulated nor induced by oxidative stress. Inactivation of or overexpression of in Sp7 resulted in an overproduction of carotenoids. It also conferred resistance to oxidative stresses and antibiotics. By controlling the synthesis of carotenoids, initiation and elongation of translation, protein folding and purine biosynthesis, RpoE2 seems to play a crucial role in preventing and repairing the cellular damage caused by oxidative stress. Lack of autoregulation and constitutive expression of suggest that RpoE2–ChrR2 may provide a rapid mechanism to cope with oxidative stress, wherein singlet oxygen (O)-mediated dissociation of the RpoE2–ChrR2 complex might release RpoE2 to drive the expression of its target genes.

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2013-02-01
2020-07-06
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