1887

Abstract

The streptolydigin biosynthetic gene cluster from NRRL 2433 contains three putative regulatory genes, , and , encoding proteins belonging to TetR and LuxR transcriptional regulator families and ATP/GTP-binding proteins of DNA and RNA helicase superfamily I, respectively. Inactivation of or resulted in the abolition of streptolydigin production, suggesting that these proteins are pathway-specific positive regulators. In the case of the mutant, low amounts of streptolydigin C were produced instead of streptolydigin. RT-PCR transcription analysis of streptolydigin biosynthesis genes revealed a hierarchical regulation process. SlgY was found to control the expression of the regulator . SlgR2 regulates the expression of structural genes involved in the formation of the streptolydigin bicyclic ketal moiety, incorporation and processing of 3-methylaspartate, and the regulator . On the other hand, SlgR1 controls the expression of , involved in the conversion of glutamate to 3-methylaspartate, and putative glycoside hydrolases and . Ectopic expression of , and regulatory genes in led to considerable increases in streptolydigin yields, 18-, 11- and 8.5-fold, respectively. Ectopic expression of in an mutant led to a 14-fold increase of streptolydigin C yields, while no effect was observed to result from expression of .

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2012-10-01
2020-07-12
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