1887

Abstract

Components of the cAMP (cyclic AMP) signalling cascades are conserved from fungi to humans, and are particularly important for fungal dimorphism and pathogenicity. Previous work has described two phosphodiesterases, UmPde1 and UmPde2, in which show strong phosphodiesterase activity. We further characterized the biological function(s) of these phosphodiesterases in . Specifically, we examined their possible role(s) in regulation of the cAMP-dependent protein kinase A (PKA) pathway and their roles in filamentous growth and pathogenicity. We found that UmPde1, which shares 35 % similarity with Pde1, also displays functional homology with this enzyme. UmPde1 complements the capsule-formation defect of strains deleted for Pde1. In , the cell morphology of the deletion mutant resembled the multiple budding phenotypes seen with the mutant, which lacks the regulatory subunit of PKA. Interestingly, on low-ammonium medium, deletion strains showed a reduction in filamentation that was comparable to that of deletion strains; however, deletion strains showed normal filamentation on low-ammonium medium. Furthermore, both the deletion strain in which the PKA pathway was constitutively active and the deletion strains were significantly reduced in pathogenicity, while the deletion strains showed a trend for reduced pathogenicity compared with wild-type strains. These data support a role for the phosphodiesterases UmPde1 and UmPde2 in regulating the cAMP-dependent PKA pathway through modulation of cAMP levels, thus affecting dimorphic growth and pathogenicity.

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2013-05-01
2019-12-06
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