1887

Abstract

Bacterial adaptation to environmental conditions is essential to ensure maximal fitness in the face of several stresses. In this context, two-component systems (TCSs) represent a predominant signal transduction mechanism, allowing an appropriate response to be mounted when a stimulus is sensed. As facultative intracellular pathogens, spp. face various environmental conditions, and an adequate response is required for a successful infection process. Recently, bioinformatic analysis of genomes predicted a set of 15 bona fide TCS pairs, among which some have been previously investigated. In this report, we characterized a new TCS locus called /, for probable proline sensor–regulator. It encodes a hybrid histidine kinase (PrlS) with an unusual Na/solute symporter N-terminal domain and a transcriptional regulator (belonging to the LuxR family) (PrlR). , spp. with a functional PrlR/S system form bacterial aggregates, which seems to be an adaptive response to a hypersaline environment, while a / mutant does not. We identified ionic strength as a possible signal sensed by this TCS. Finally, this work correlates the absence of a functional PrlR/S system with the lack of hypersaline-induced aggregation in particular marine spp.

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2012-10-01
2022-01-29
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