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Volume 158, Issue 10

The / gene encodes a low temperature-induced Dps protein homologue in the cyanobacteria sp. PCC 7120 and M3 Free

Abstract

DNA-binding proteins from starved cells (Dps), which are encoded by many bacterial genomes, protect genomic DNA via non-specific DNA binding, as well as inhibition of free radical formation by chelating Fe(II). In the filamentous cyanobacterium , the second gene () in the low temperature-induced gene operon in strain M3 was found to encode a homologue of Dps, but for a long time this gene remained poorly characterized. A gene cluster, ––, was found later to be 100 % identical to the gene cluster in a closely related strain, PCC 7120. In the present study, we detected ferroxidase activity of the Lti46.2/All0458 protein, which formed a dodecamer, as found in other Dps proteins. In addition, three homologues of were found in strain PCC 7120, namely, , and . We analysed expression of the or gene cluster in both strains, M3 and PCC 7120, and confirmed its induction by low temperature. We found that the All0458–GFP fusion protein and the All1173–GFP fusion protein were localized to the nucleoids. In the null mutant, the transcript of the gene accumulated. These results suggest that there might be complex cooperation of various members of the family in protecting the genome from environmental stresses such as changing temperature.

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2012-10-01
2024-04-20
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The all0458/lti46.2 gene encodes a low temperature-induced Dps protein homologue in the cyanobacteria Anabaena sp. PCC 7120 and Anabaena variabilis M3
Microbiology 158, 2527 (2012); https://doi.org/10.1099/mic.0.060657-0
/content/journal/micro/10.1099/mic.0.060657-0
/content/journal/micro/10.1099/mic.0.060657-0
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