The gene is involved in the stress response of during vegetative growth and development Free

Abstract

The Clp/HSP100 family of molecular chaperones is ubiquitous in both prokaryotes and eukaryotes. These proteins play important roles in refolding, disaggregating and degrading proteins damaged by stress. As a subclass of the Clp/HSP100 family, ClpB has been shown to be involved in various stress responses as well as other functions in bacteria. In the present study, we investigated the role of a predicted ClpB-encoding gene, , in the stress response during vegetative growth and development of . Transcriptional analysis confirmed induction of this homologue under different stress conditions, and further phenotypic analysis revealed that an in-frame deletion mutant of was more sensitive to various stress treatments than the wild-type strain during vegetative growth. Moreover, the absence of the gene resulted in decreased heat tolerance of myxospores, indicating the involvement of this homologue in the stress response during the development of myxospores. The recombinant ClpB (MXAN5092) protein also showed a general chaperone activity . Overall, our genetic and phenotypic analysis of the predicted ATP-dependent chaperone protein ClpB (MXAN5092) demonstrated that it functions as a chaperone protein and plays an important role in cellular stress tolerance during both vegetative growth and development of .

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2012-09-01
2024-03-29
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