1887

Abstract

Protein-tyrosine phosphorylation plays a significant role in multiple cellular functions in bacteria. Bacterial tyrosine phosphatases catalyse the dephosphorylation of tyrosyl-phosphorylated proteins. PhpA shares homology with DNA polymerase and histidinol phosphatase family members. Recombinant His-tagged PhpA requires Mn or Co for phosphatase activity, and shows strict specificity for phosphorylated tyrosine residues. The values of PhpA for -nitrophenyl phosphate (NPP) and phosphotyrosine peptide, RRLIEDAEpYAARG, were 803 and 139 µM, respectively. The phosphatase activity of PhpA was inhibited by sodium orthovanadate with a of 33 µM. gene expression was observed under both vegetative and developmental conditions, but peaked during late fruiting body formation. A mutant exhibited an elevated level of tyrosine phosphorylation of a 79 kDa protein and cytoplasmic tyrosine kinase, BtkA. In , exopolysaccharide (EPS) is essential for cell–cell adhesion and fruiting body formation. mutant cells exhibited enhanced capacity for cell–cell agglutination in agglutination buffer. Under starvation conditions, mutation caused early aggregation and sporulation. The EPS production assay showed that the mutant produced an increased amount of EPS in comparison with the wild-type. These results indicate that PhpA may negatively regulate the production of EPS in .

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2012-10-01
2019-12-08
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