1887

Abstract

, originally isolated from honeybee intestine, was found to grow under 20 % O conditions in liquid shaking culture using MRS broth. Catalase activity was detected only in cells that were exposed to O and grown in medium containing a haem source, and these cells showed higher viability on exposure to HO. Passage through multiple column chromatography steps enabled purification of the active protein, which was identified as a homologue of haem catalase on the basis of its N-terminal sequence. The enzyme is a homodimer composed of a subunit with a molecular mass of 55 kDa, and the absorption spectrum shows the typical profile of bacterial haem catalase. A gene encoding haem catalase, which has an amino acid sequence coinciding with the N-terminal amino acid sequence of the purified protein, was found in the draft genome sequence data of . Expression of the gene was induced in response to O exposure. The haem catalase from shows about 70–80 % identity with those from lactobacilli and other lactic acid bacteria, and no homologues were found in other bifidobacterial genomes.

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2013-01-01
2019-10-19
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