1887

Abstract

We determined the ability of to utilize enterobactin (Ent) as a xenosiderophore. Homology searches of the genomic sequence revealed the presence of genes that are homologous to the ferric Ent utilization genes, which consist of the iron-repressible outer-membrane protein genes and , and the ATP-binding cassette transport system operon . Moreover, the and genes, which encode transcriptional regulators, were also found immediately upstream of and , respectively. Growth assays of indicated that both and mutants grew well in the presence of Ent under iron-limiting conditions, whereas both the / double mutant and the mutant barely grew under the same conditions. In addition, growth assays of three isogenic mutants demonstrated that the TonB2 system, and to a lesser extent the TonB1 system, can provide energy for both IrgA and VctA to transport ferric Ent. SDS-PAGE analysis showed that expression of both IrgA and VctA was enhanced by the presence of Ent. Complementation of the and mutants with their respective genes resulted in the increased expression of IrgA and VctA, respectively. Finally, reverse transcriptase-quantitative PCR revealed that transcription of the Ent utilization system genes is iron-regulated, and that transcription of and under iron-limiting conditions is further activated by proteins encoded by and , respectively, together with Ent.

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2012-08-01
2021-10-16
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