1887

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Volume 158, Issue 12

, a Hog1-type mitogen-activated protein kinase gene, contributes to stress tolerance and virulence of the entomopathogenic fungus Free

Abstract

Fungal biocontrol agents have great potential in integrated pest management. However, poor efficacy and sensitivity to various adverse factors have hampered their wide application. In eukaryotic cells, Hog1 kinase plays a critical role in stress responses. In this study, (GenBank accession no. EFY85878), encoding a member of the Hog1/Sty1/p38 mitogen-activated protein kinase family in () , was identified. Targeted gene disruption was used to analyse the role of in virulence and tolerance of adverse factors. Mutants with depletion showed increased sensitivity to high osmotic stress, high temperature and oxidative stress, and exhibited remarkable resistance to cell wall-disturbing agents. These results suggest that Hog1 kinase has a conserved function in regulating multistress responses among fungi, and that might influence cell wall biogenesis in . Bioassays conducted with topical inoculation and intrahaemocoel injection revealed that is required for both penetration and postpenetration development of . disruption resulted in a significant reduction in virulence, likely due to the combination of a decrease in conidial germination, a reduction in appressorium formation and a decline in growth rate in insect haemolymph, which might be caused by impairing fungal tolerance of various stresses during infection.

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  • Accepted:
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2012-12-01
2024-04-19
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MaHog1, a Hog1-type mitogen-activated protein kinase gene, contributes to stress tolerance and virulence of the entomopathogenic fungus Metarhizium acridum
Microbiology 158, 2987 (2012); https://doi.org/10.1099/mic.0.059469-0
/content/journal/micro/10.1099/mic.0.059469-0
/content/journal/micro/10.1099/mic.0.059469-0
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