RT Journal Article SR Electronic(1) A1 Brouwers, Erin A1 Ma, Irene A1 Thomas, Nikhil A.YR 2012 T1 Dual temporal transcription activation mechanisms control cesT expression in enteropathogenic Escherichia coli JF Microbiology, VO 158 IS 9 SP 2246 OP 2261 DO https://doi.org/10.1099/mic.0.059444-0 PB Microbiology Society, SN 1465-2080, AB The locus of enterocyte effacement (LEE) is a 35 kb pathogenicity island involved in attaching and effacing (A/E) Escherichia coli enteric infection. The LEE is organized into five large transcriptional operons (LEE1–LEE5) and a few bi- and monocistronic instances. The LEE5 operon co-transcribes three genes, tir-cesT-eae, although cesT can be transcribed in a separate mRNA from its own proximal promoter. The role of this separate promoter is not understood. In this study we characterized promoter activity for the type III secretion chaperone gene cesT. The cesT promoter, cesTp, has features consistent with σ70 promoters that contain an extended −10 element. This was experimentally confirmed by mutations that altered cesTp activity. In stark contrast to LEE2–5 transcriptional operons, cesTp did not require the master regulator Ler for transcriptional activity. Moreover, cesTp activity was not dependent on the presence of GrlA or GrlR, two regulators associated with LEE gene expression. A cesTp–lux fusion was used in real-time assays and demonstrated initial cesTp activity that occurred before LEE5 promoter activity, which ensued after 120 min. cesTp was shown to be active during in vitro infection of HT-29 colonic epithelial cells. Inactivation of cesTp reduced CesT protein levels at early growth time points. These data indicate a Ler-, GrlA- and GrlR-independent activity for cesTp. We suggest that A/E pathogenic E. coli have evolved a mechanism to ready the cell for CesT protein expression in support of infection prior to Ler- and GrlA-related activities., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.059444-0