1887

Abstract

A single polar flagellum and motility are potential virulence factors of , a foodborne pathogen. In the present study, the functions of FlhF and regulatory characteristics of the expression of were investigated. A deletion mutation in abolished motility, flagella formation and flagellin synthesis, and introduction of complemented the defects. The mutant revealed decreased expression of the class III and IV flagella genes, indicating that FlhF is a key regulator for the flagellar biogenesis of . The influence of global regulatory proteins on the expression of was examined and SmcR, a LuxR homologue, was found to downregulate expression at the transcriptional level. SmcR represses expression only in the stationary phase of growth and exerts its effects by directly binding to the promoter region. Finally, an SmcR binding site, centred at 22.5 bp upstream of the transcription start site, was identified by a DNase I protection assay. The combined results demonstrate that a quorum sensing master regulator SmcR influences the motility and flagellar biogenesis of through modulating the expression of FlhF in a growth-phase-dependent manner.

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2012-08-01
2020-01-25
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