1887

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Volume 158, Issue 6

Influence of the ADP/ATP ratio, 2-oxoglutarate and divalent ions on P protein signalling Free

Abstract

Proteins belonging to the P family coordinate cellular nitrogen metabolism by direct interaction with a variety of enzymes, transcriptional regulators and transporters. The sensing function of P relies on its ability to bind the nitrogen/carbon signalling molecule 2-oxoglutarate (2-OG). In Proteobacteria, P is further subject to reversible uridylylation according to the intracellular levels of glutamine, which reflect the cellular nitrogen status. A number of P proteins have been shown to bind ADP and ATP in a competitive manner, suggesting that P might act as an energy sensor. Here, we analyse the influence of the ADP/ATP ratio, 2-OG levels and divalent metal ions on uridylylation of the P proteins GlnB and GlnZ, and on interaction with their targets AmtB, DraG and DraT. The results support the notion that the cellular concentration of 2-OG is a key factor governing occupation of the GlnB and GlnZ nucleotide binding sites by ATP or ADP, with high 2-OG levels favouring the occupation of P by ATP. Both P uridylylation and interaction with target proteins responded to the ADP/ATP ratio within the expected physiological range, supporting the concept that P proteins might act as cellular energy sensors.

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2012-06-01
2024-04-24
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Influence of the ADP/ATP ratio, 2-oxoglutarate and divalent ions on Azospirillum brasilense PII protein signalling
Microbiology 158, 1656 (2012); https://doi.org/10.1099/mic.0.058446-0
/content/journal/micro/10.1099/mic.0.058446-0
/content/journal/micro/10.1099/mic.0.058446-0
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