1887

Abstract

The BasS–BasR two-component system is known as an iron- and zinc-sensing transcription regulator in , but so far only a few genes have been identified to be under the direct control of phosphorylated BasR. Using Genomic SELEX (systematic evolution of ligands by exponential enrichment) screening, we have identified a total of at least 38 binding sites of phosphorylated BasR on the genome, and based on the BasR-binding sites, have predicted more than 20 novel targets of regulation. By DNase I footprint analysis for high-affinity BasR-binding sites, a direct repeat of a TTAAnnTT sequence was identified as the BasR box. Transcription regulation of the target genes was confirmed after Northern blot analysis of target gene mRNAs from both wild-type and an otherwise isogenic deletion mutant. The BasR regulon can be classified into three groups of genes: group 1 includes the genes for the formation and modification of membrane structure; group 2 includes genes for modulation of membrane functions; and group 3 includes genes for stress-response cell functions, including , the master regulator of biofilm formation.

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2012-06-01
2024-12-12
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