@article{mbs:/content/journal/micro/10.1099/mic.0.057448-0, author = "Han, Huiming and Liu, Cuihua and Wang, Quanhui and Xuan, Chunling and Zheng, Beiwen and Tang, Jiaqi and Yan, Jinghua and Zhang, Jingren and Li, Ming and Cheng, Hao and Lu, Guangwen and Gao, George F.", title = "The two-component system Ihk/Irr contributes to the virulence of Streptococcus suis serotype 2 strain 05ZYH33 through alteration of the bacterial cell metabolism", journal= "Microbiology", year = "2012", volume = "158", number = "7", pages = "1852-1866", doi = "https://doi.org/10.1099/mic.0.057448-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.057448-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen. It causes heavy economic losses in the pig-farming industry and can be associated with severe infections in humans, e.g. streptococcal toxic shock syndrome. Understanding its pathogenesis is critical for prevention and control of diseases caused by S. suis 2. In this study, we show that deletion of a two-component system (TCS), 05SSU1660/1661 (orthologues of the Ihk/Irr TCS of Streptococcus pyogenes), in S. suis 2 strain 05ZYH33 results in notable attenuation of virulence, as exemplified by reduced adherence to mucosal epithelium cells, increased elimination by macrophages, reduced ability to survive in an acidic or oxidative-stressed environment, and lowered pathogenicity in mice. Further analysis of differential proteomics profiles by two-dimensional electrophoresis revealed that while many previously well-known virulence factors, such as suilysin, autolysin and muraminidase-released protein, were not expressed differentially, cell metabolism was downregulated in the Ihk/Irr deletion mutant. In addition, the oxidative-stress response gene for manganese-dependent superoxide dismutase (MnSOD) was also repressed significantly in the mutant. Collectively, our data suggest that the Ihk/Irr TCS contributes to the virulence of S. suis 2 strain 05ZYH33, mainly through alteration of the bacterial cell metabolism.", }