1887

Abstract

Although and serovar Typhimurium have a similar flagellar regulatory system, the response of flagellar synthesis to nutrient conditions is quite different between the two: that is, in low-nutrient conditions, flagellar synthesis is inhibited in and enhanced in . In , this inhibition is mediated by an anti-FlhDC factor, YdiV, which is expressed in low-nutrient conditions and binds to FlhDC to inhibit the expression of the class 2 flagellar genes. The gene encodes a repressor of the gene, and thus is required for efficient flagellar gene expression in low-nutrient conditions in . In this study, we showed that the . gene encodes a protein which inhibits motility and flagellar production when expressed from a multicopy plasmid. We showed further that . YdiV binds to FlhDC and inhibits its binding to the class 2 flagellar promoter. These results indicate that . YdiV can also act as an anti-FlhDC factor. However, although the gene was transcribed efficiently in cells, the intracellular level of the YdiV protein was extremely low due to its inefficient translation. Consistent with this, cells did not require FliZ for efficient motility development. This indicates that, unlike in , the FliZ–YdiV regulatory system does not work in the nutritional control of flagellar gene expression in .

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2012-06-01
2019-10-18
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