RT Journal Article SR Electronic(1) A1 Reffuveille, Fany A1 Serror, Pascale A1 Chevalier, Sylvie A1 Budin-Verneuil, Aurélie A1 Ladjouzi, Rabia A1 Bernay, Benoit A1 Auffray, Yanick A1 Rincé, AlainYR 2012 T1 The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence JF Microbiology, VO 158 IS 3 SP 816 OP 825 DO https://doi.org/10.1099/mic.0.055319-0 PB Microbiology Society, SN 1465-2080, AB Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn2+ or under oxidative stress in vitro, and significantly decreased virulence., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.055319-0