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Volume 158, Issue 2

Discovery of DNA operators for TetR and MarR family transcription factors from Free

Abstract

Determining transcription factor (TF) recognition motifs or operator sites is central to understanding gene regulation, yet few operators have been characterized. In this study, we used a protein-binding microarray (PBM) to discover the DNA recognition sites and putative regulons for three TetR and one MarR family TFs derived from , which are common to the genus . We also describe the development and application of a more streamlined version of the PBM technology that significantly reduced the experimental time. Despite the genus containing many pathogenically important species, only a handful of TF operator sites have been experimentally characterized for to date. Our study provides a significant addition to this knowledge base and illustrates some general challenges of discovering operators on a large scale for prokaryotes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 2012 SGM
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2012-02-01
2024-04-25
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Discovery of DNA operators for TetR and MarR family transcription factors from Burkholderia xenovorans
Microbiology 158, 571 (2012); https://doi.org/10.1099/mic.0.055129-0
/content/journal/micro/10.1099/mic.0.055129-0
/content/journal/micro/10.1099/mic.0.055129-0
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