1887

Abstract

Factor H binding protein (fHbp) is a major antigenic component of novel vaccines designed to protect against meningococcal disease. Prediction of the potential coverage of these vaccines is difficult, as fHbp is antigenically variable and levels of expression differ among isolates. Transcriptional regulation of the gene is poorly understood, although evidence suggests that oxygen availability is involved. In this study iron accessibility was found to affect transcription. However, regulation differed among meningococcal clonal complexes (ccs). For the majority of isolates, increased iron concentrations upregulated transcription. This effect was enhanced by the presence of a 181 bp insertion element upstream of , associated with isolates belonging to cc4 and cc5. Conversely, meningococci belonging to cc32 showed iron-repressed control of , as regulation was dominated by cotranscription with the iron-repressed upstream gene . These results highlight the complexity of regulation and demonstrate that control of transcription can vary among genetic lineages.

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2012-04-01
2021-07-27
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