1887

Abstract

In only one essential oligoribonuclease (Orn) can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). In NrnA and NrnB, which do not show any sequence similarity to Orn, have been identified as functional analogues of Orn. Sequence comparisons did not identify , or homologues in the genomes of the / and family members. Screening a genomic library from a member of the , for genes that can complement a conditional mutant in , we identified BA0969 (NrnC) as a functional analogue of Orn. NrnC is highly conserved (more than 80 % identity) in the genomes sequenced to date. Biochemical characterization showed that this protein exhibits oligo RNA degradation activity (nanoRNase activity). Like Orn from , NrnC is inhibited by micromolar amounts of 3′-phosphoadenosine 5′-phosphate . NrnC homologues are widely present in genomes of . Knock down of decreases the growth ability of , demonstrating the importance of nanoRNase activity in this bacterium.

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2012-04-01
2019-10-19
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