RT Journal Article SR Electronic(1) A1 Gaballa, Ahmed A1 Helmann, John D.YR 2011 T1 Bacillus subtilis Fur represses one of two paralogous haem-degrading monooxygenases JF Microbiology, VO 157 IS 11 SP 3221 OP 3231 DO https://doi.org/10.1099/mic.0.053579-0 PB Microbiology Society, SN 1465-2080, AB Identification of genes regulated by the ferric uptake regulator (Fur) protein has provided insights into the diverse mechanisms of adaptation to iron limitation. In the soil bacterium Bacillus subtilis, Fur senses iron sufficiency and represses genes that enable iron uptake, including biosynthetic and transport genes for the siderophore bacillibactin and uptake systems for siderophores produced by other organisms. We here demonstrate that Fur regulates hmoA (formerly yetG), which encodes a haem monooxygenase. HmoA is the first characterized member of a divergent group of putative monooxygenases that cluster separately from the well-characterized IsdG family. B. subtilis also encodes an IsdG family protein designated HmoB (formerly YhgC). Unlike hmoA, hmoB is constitutively expressed and not under Fur control. HmoA and HmoB both bind haemin in vitro with approximately 1 : 1 stoichiometry and degrade haemin in the presence of an electron donor. Mutational and spectroscopic analyses indicate that HmoA and HmoB have distinct active site architectures and interact differently with haem. We further show that B. subtilis can use haem as an iron source, but that this ability is independent of HmoA and HmoB., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.053579-0