1887

Abstract

We have identified a methanol- and biotin-starvation-inducible zinc finger protein named ROP [repressor of phosphoenolpyruvate carboxykinase (PEPCK)] in the methylotrophic yeast . When strain GS115 (wild-type, WT) is cultured in biotin-deficient, glucose-ammonium (Bio) medium, growth is suppressed due to the inhibition of anaplerotic synthesis of oxaloacetate, catalysed by the biotin-dependent enzyme pyruvate carboxylase (PC). Deletion of results in a strain (Δ) that can grow under biotin-deficient conditions due to derepression of a biotin- and PC-independent pathway of anaplerotic synthesis of oxaloacetate. Northern analysis as well as microarray expression profiling of RNA isolated from WT and Δ strains cultured in Bio medium indicate that expression of the phosphoenolpyruvate carboxykinase gene () is induced in Δ during biotin- or PC-deficiency even under glucose-abundant conditions. There is an excellent correlation between expression and growth of Δ in Bio medium, suggesting that ROP-mediated regulation of may have a crucial role in the biotin- and PC-independent growth of the Δ strain. To our knowledge, ROP is the first example of a zinc finger transcription factor involved in the catabolite repression of in yeast cells cultured under biotin- or PC-deficient and glucose-abundant conditions.

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2011-12-01
2020-10-28
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