1887

Abstract

The carbazole-degradative plasmid pCAR1 carries the class II transposon Tn, which contains the and genes, essential for conversion of carbazole into anthranilate, and anthranilate into catechol, respectively. In our previous study, DNA rearrangements in pCAR1 were frequently detected in the host Pf0-1 in the presence of carbazole, resulting in the improvement of host survivability. Several Pf0-1 mutants harbouring pCAR1 were isolated, and deletion of DNA in the plasmid gene was found. Here, we compared genome sequences of the parent strain Pf0-1L(pCAR1 : : ) and one of its mutants, 5EP83, to assess whether other DNA rearrangements occurred in either the plasmid or the host chromosome. We found transposition of Tn into the 5EP83 chromosome. In addition, IS had transposed into the gene intergenic region on the pCAR1-derivative plasmid of 5EP83, which inhibited transcription. As a result of these transpositions, 5EP83 was able to metabolize carbazole due to the Tn on its chromosome, although the genes on its plasmid were non-functional. We also found that one copy of duplicate genes had been deleted, and that IS had transposed into both the host chromosome and the plasmid. Our findings suggest that Pf0-1 harbouring pCAR1 is subjected to DNA rearrangements not only on the plasmid but also on its chromosome in the presence of carbazole.

Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology (Award S0801025)
  • RIKEN
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2011-12-01
2021-10-25
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