1887

Abstract

() is a south-east Asian tree more commonly known as ‘Jarul’. Research on health benefits suggests that the . plant contains phytomolecules that may have antioxidant, anti-diabetic and anti-obesity properties. However, antimicrobial activities have not been reported for this plant. The ability of fruit extract (LSFE) to antagonize cell-to-cell communication, expression of virulence genes and factors, and biofilm formation was evaluated in strain PAO1. Our results suggested that LSFE caused downregulation of quorum sensing (QS)-related genes ( and ) and their respective signalling molecules, -acylhomoserine lactones, without affecting the growth of PAO1. Significant inhibition of virulence factors: LasA protease, LasB elastase, and pyoverdin production, was also recorded. Application of LSFE to PAO1 biofilms increased bacterial susceptibility to tobramycin. These data suggest a possible role for quorum-quenching mechanisms unrelated to static or cidal effects, and also suggest that . could serve as a cost-effective source in the development of new QS-based antibacterial drugs.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.052985-0
2012-02-01
2022-01-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/158/2/529.html?itemId=/content/journal/micro/10.1099/mic.0.052985-0&mimeType=html&fmt=ahah

References

  1. Adonizio A. L., Downum K., Bennett B. C., Mathee K. ( 2006). Anti-quorum sensing activity of medicinal plants in southern Florida. J Ethnopharmacol 105:427–435 [View Article][PubMed]
    [Google Scholar]
  2. Adonizio A., Kong K. F., Mathee K. ( 2008). Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Chemother 52:198–203 [View Article][PubMed]
    [Google Scholar]
  3. Atkinson S., Throup J. P., Stewart G. S., Williams P. ( 1999). A hierarchical quorum-sensing system in Yersinia pseudotuberculosis is involved in the regulation of motility and clumping. Mol Microbiol 33:1267–1277 [View Article][PubMed]
    [Google Scholar]
  4. Bergstrom L. C., Qin L., Harlocker S. L., Egger L. A., Inouye M. ( 1998). Hierarchical and co-operative binding of OmpR to a fusion construct containing the ompC and ompF upstream regulatory sequences of Escherichia coli . Genes Cells 3:777–788 [View Article][PubMed]
    [Google Scholar]
  5. Bever R. A., Iglewski B. H. ( 1988). Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene. J Bacteriol 170:4309–4314[PubMed]
    [Google Scholar]
  6. Bosgelmez-Tinaz G., Ulusoy S., Ugur A., Ceylan O. ( 2007). Inhibition of quorum sensing-regulated behaviors by Scorzonera sandrasica . Curr Microbiol 55:114–118 [View Article][PubMed]
    [Google Scholar]
  7. Brint J. M., Ohman D. E. ( 1995). Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J Bacteriol 177:7155–7163[PubMed]
    [Google Scholar]
  8. Carter G. P., Purdy D., Williams P., Minton N. P. ( 2005). Quorum sensing in Clostridium difficile: analysis of a luxS-type signalling system. J Med Microbiol 54:119–127 [View Article][PubMed]
    [Google Scholar]
  9. Choo J. H., Rukayadi Y., Hwang J. K. ( 2006). Inhibition of bacterial quorum sensing by vanilla extract. Lett Appl Microbiol 42:637–641[PubMed]
    [Google Scholar]
  10. Cox C. D., Adams P. ( 1985). Siderophore activity of pyoverdin for Pseudomonas aeruginosa . Infect Immun 48:130–138[PubMed]
    [Google Scholar]
  11. Davies D. G., Parsek M. R., Pearson J. P., Iglewski B. H., Costerton J. W., Greenberg E. P. ( 1998). The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295–298 [View Article][PubMed]
    [Google Scholar]
  12. Dong Y. H., Wang L. H., Xu J. L., Zhang H. B., Zhang X. F., Zhang L. H. ( 2001). Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase. Nature 411:813–817 [View Article][PubMed]
    [Google Scholar]
  13. Eberhard A., Burlingame A. L., Eberhard C., Kenyon G. L., Nealson K. H., Oppenheimer N. J. ( 1981). Structural identification of autoinducer of Photobacterium fischeri luciferase. Biochemistry 20:2444–2449 [View Article][PubMed]
    [Google Scholar]
  14. Fuqua C., Greenberg E. P. ( 2002). Listening in on bacteria: acyl-homoserine lactone signalling. Nat Rev Mol Cell Biol 3:685–695 [View Article][PubMed]
    [Google Scholar]
  15. Gao M., Teplitski M., Robinson J. B., Bauer W. D. ( 2003). Production of substances by Medicago truncatula that affect bacterial quorum sensing. Mol Plant Microbe Interact 16:827–834 [View Article][PubMed]
    [Google Scholar]
  16. Givskov M., de Nys R., Manefield M., Gram L., Maximilien R., Eberl L., Molin S., Steinberg P. D., Kjelleberg S. ( 1996). Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling. J Bacteriol 178:6618–6622[PubMed]
    [Google Scholar]
  17. Glessner A., Smith R. S., Iglewski B. H., Robinson J. B. ( 1999). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. J Bacteriol 181:1623–1629[PubMed]
    [Google Scholar]
  18. Grossman A. D. ( 1995). Genetic networks controlling the initiation of sporulation and the development of genetic competence in Bacillus subtilis . Annu Rev Genet 29:477–508 [View Article][PubMed]
    [Google Scholar]
  19. Hentzer M., Givskov M. ( 2003). Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. J Clin Invest 112:1300–1307[PubMed] [CrossRef]
    [Google Scholar]
  20. Hentzer M., Riedel K., Rasmussen T. B., Heydorn A., Andersen J. B., Parsek M. R., Rice S. A., Eberl L., Molin S. & other authors ( 2002). Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148:87–102[PubMed]
    [Google Scholar]
  21. Huber B., Eberl L., Feucht W., Polster J. ( 2003). Influence of polyphenols on bacterial biofilm formation and quorum-sensing. Z Naturforsch C 58:879–884[PubMed] [CrossRef]
    [Google Scholar]
  22. Keshavan N. D., Chowdhary P. K., Haines D. C., González J. E. ( 2005). l-Canavanine made by Medicago sativa interferes with quorum sensing in Sinorhizobium meliloti . J Bacteriol 187:8427–8436 [View Article][PubMed]
    [Google Scholar]
  23. Kessler E. ( 1995). β-Lytic endopeptidases. Methods Enzymol 248:740–756 [View Article][PubMed]
    [Google Scholar]
  24. Kessler E., Safrin M., Gustin J. K., Ohman D. E. ( 1998). Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides. J Biol Chem 273:30225–30231 [View Article][PubMed]
    [Google Scholar]
  25. Kjelleberg S., Molin S. ( 2002). Is there a role for quorum sensing signals in bacterial biofilms?. Curr Opin Microbiol 5:254–258 [View Article][PubMed]
    [Google Scholar]
  26. Kong K. F., Jayawardena S. R., Indulkar S. D., Del Puerto A., Koh C. L., Høiby N., Mathee K. ( 2005). Pseudomonas aeruginosa AmpR is a global transcriptional factor that regulates expression of AmpC and PoxB β-lactamases, proteases, quorum sensing, and other virulence factors. Antimicrob Agents Chemother 49:4567–4575 [View Article][PubMed]
    [Google Scholar]
  27. Lutter E., Lewenza S., Dennis J. J., Visser M. B., Sokol P. A. ( 2001). Distribution of quorum-sensing genes in the Burkholderia cepacia complex. Infect Immun 69:4661–4666 [View Article][PubMed]
    [Google Scholar]
  28. Lyon G. J., Muir T. W. ( 2003). Chemical signaling among bacteria and its inhibition. Chem Biol 10:1007–1021 [View Article][PubMed]
    [Google Scholar]
  29. Manefield M., Rasmussen T. B., Henzter M., Andersen J. B., Steinberg P., Kjelleberg S., Givskov M. ( 2002). Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover. Microbiology 148:1119–1127[PubMed]
    [Google Scholar]
  30. Mathee K., McPherson C. J., Ohman D. E. ( 1997). Posttranslational control of the algT (algU)-encoded σ22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN). J Bacteriol 179:3711–3720[PubMed]
    [Google Scholar]
  31. Meyer J. M., Neely A., Stintzi A., Georges C., Holder I. A. ( 1996). Pyoverdin is essential for virulence of Pseudomonas aeruginosa . Infect Immun 64:518–523[PubMed]
    [Google Scholar]
  32. Mittal R., Sharma S., Chhibber S., Harjai K. ( 2008). Iron dictates the virulence of Pseudomonas aeruginosa in urinary tract infections. J Biomed Sci 15:731–741 [View Article][PubMed]
    [Google Scholar]
  33. O’Toole G. A., Kolter R. ( 1998). Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol 28:449–461 [View Article][PubMed]
    [Google Scholar]
  34. Ohman D. E., Cryz S. J., Iglewski B. H. ( 1980). Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. J Bacteriol 142:836–842[PubMed]
    [Google Scholar]
  35. Parsek M. R., Greenberg E. P. ( 2000). Acyl-homoserine lactone quorum sensing in Gram-negative bacteria: a signaling mechanism involved in associations with higher organisms. Proc Natl Acad Sci U S A 97:8789–8793 [View Article][PubMed]
    [Google Scholar]
  36. Rasch M., Buch C., Austin B., Slierendrecht W. J., Ekmann K. S., Larsen J. L., Johansen C., Riedel K., Eberl L. & other authors ( 2004). An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). Syst Appl Microbiol 27:350–359 [View Article][PubMed]
    [Google Scholar]
  37. Rasmussen T. B., Bjarnsholt T., Skindersoe M. E., Hentzer M., Kristoffersen P., Köte M., Nielsen J., Eberl L., Givskov M. ( 2005). Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol 187:1799–1814 [View Article][PubMed]
    [Google Scholar]
  38. Ren D., Sims J. J., Wood T. K. ( 2002). Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone. Lett Appl Microbiol 34:293–299 [View Article][PubMed]
    [Google Scholar]
  39. Rodelas B., Lithgow J. K., Wisniewski-Dye F., Hardman A., Wilkinson A., Economou A., Williams P., Downie J. A. ( 1999). Analysis of quorum-sensing-dependent control of rhizosphere-expressed (rhi) genes in Rhizobium leguminosarum bv. viciae . J Bacteriol 181:3816–3823[PubMed]
    [Google Scholar]
  40. Schuster M., Greenberg E. P. ( 2006). A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa . Int J Med Microbiol 296:73–81 [View Article][PubMed]
    [Google Scholar]
  41. Shaw P. D., Ping G., Daly S. L., Cha C., Cronan J. E. Jr, Rinehart K. L., Farrand S. K. ( 1997). Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc Natl Acad Sci U S A 94:6036–6041 [View Article][PubMed]
    [Google Scholar]
  42. Singh B. N., Singh B. R., Singh R. L., Prakash D., Dhakarey R., Upadhyay G., Singh H. B. ( 2009a). Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera . Food Chem Toxicol 47:1109–1116 [View Article][PubMed]
    [Google Scholar]
  43. Singh B. N., Singh B. R., Singh R. L., Prakash D., Sarma B. K., Singh H. B. ( 2009b). Antioxidant and anti-quorum sensing activities of green pod of Acacia nilotica L. Food Chem Toxicol 47:778–786 [View Article][PubMed]
    [Google Scholar]
  44. Sitnikov D. M., Schineller J. B., Baldwin T. O. ( 1995). Transcriptional regulation of bioluminescence genes from Vibrio fischeri . Mol Microbiol 17:801–812 [View Article][PubMed]
    [Google Scholar]
  45. Surette M. G., Miller M. B., Bassler B. L. ( 1999). Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. Proc Natl Acad Sci U S A 96:1639–1644 [View Article][PubMed]
    [Google Scholar]
  46. Teplitski M., Robinson J. B., Bauer W. D. ( 2000). Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. Mol Plant Microbe Interact 13:637–648 [View Article][PubMed]
    [Google Scholar]
  47. Venturi V. ( 2006). Regulation of quorum sensing in Pseudomonas . FEMS Microbiol Rev 30:274–291 [View Article][PubMed]
    [Google Scholar]
  48. Von Bodman S. B., Bauer W. D., Coplin D. L. ( 2003). Quorum sensing in plant-pathogenic bacteria. Annu Rev Phytopathol 41:455–482 [View Article][PubMed]
    [Google Scholar]
  49. Whitehead N. A., Welch M., Salmond G. P. ( 2001). Silencing the majority. Nat Biotechnol 19:735–736 [View Article][PubMed]
    [Google Scholar]
  50. Zhu H., Sun S. J. ( 2008). Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Curr Microbiol 57:418–422 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.052985-0
Loading
/content/journal/micro/10.1099/mic.0.052985-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error