1887

Abstract

() is a south-east Asian tree more commonly known as ‘Jarul’. Research on health benefits suggests that the . plant contains phytomolecules that may have antioxidant, anti-diabetic and anti-obesity properties. However, antimicrobial activities have not been reported for this plant. The ability of fruit extract (LSFE) to antagonize cell-to-cell communication, expression of virulence genes and factors, and biofilm formation was evaluated in strain PAO1. Our results suggested that LSFE caused downregulation of quorum sensing (QS)-related genes ( and ) and their respective signalling molecules, -acylhomoserine lactones, without affecting the growth of PAO1. Significant inhibition of virulence factors: LasA protease, LasB elastase, and pyoverdin production, was also recorded. Application of LSFE to PAO1 biofilms increased bacterial susceptibility to tobramycin. These data suggest a possible role for quorum-quenching mechanisms unrelated to static or cidal effects, and also suggest that . could serve as a cost-effective source in the development of new QS-based antibacterial drugs.

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2012-02-01
2019-10-20
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References

  1. Adonizio A. L. , Downum K. , Bennett B. C. , Mathee K. . ( 2006; ). Anti-quorum sensing activity of medicinal plants in southern Florida. . J Ethnopharmacol 105:, 427–435. [CrossRef] [PubMed]
    [Google Scholar]
  2. Adonizio A. , Kong K. F. , Mathee K. . ( 2008; ). Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. . Antimicrob Agents Chemother 52:, 198–203. [CrossRef] [PubMed]
    [Google Scholar]
  3. Atkinson S. , Throup J. P. , Stewart G. S. , Williams P. . ( 1999; ). A hierarchical quorum-sensing system in Yersinia pseudotuberculosis is involved in the regulation of motility and clumping. . Mol Microbiol 33:, 1267–1277. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bergstrom L. C. , Qin L. , Harlocker S. L. , Egger L. A. , Inouye M. . ( 1998; ). Hierarchical and co-operative binding of OmpR to a fusion construct containing the ompC and ompF upstream regulatory sequences of Escherichia coli . . Genes Cells 3:, 777–788. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bever R. A. , Iglewski B. H. . ( 1988; ). Molecular characterization and nucleotide sequence of the Pseudomonas aeruginosa elastase structural gene. . J Bacteriol 170:, 4309–4314.[PubMed]
    [Google Scholar]
  6. Bosgelmez-Tinaz G. , Ulusoy S. , Ugur A. , Ceylan O. . ( 2007; ). Inhibition of quorum sensing-regulated behaviors by Scorzonera sandrasica . . Curr Microbiol 55:, 114–118. [CrossRef] [PubMed]
    [Google Scholar]
  7. Brint J. M. , Ohman D. E. . ( 1995; ). Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. . J Bacteriol 177:, 7155–7163.[PubMed]
    [Google Scholar]
  8. Carter G. P. , Purdy D. , Williams P. , Minton N. P. . ( 2005; ). Quorum sensing in Clostridium difficile: analysis of a luxS-type signalling system. . J Med Microbiol 54:, 119–127. [CrossRef] [PubMed]
    [Google Scholar]
  9. Choo J. H. , Rukayadi Y. , Hwang J. K. . ( 2006; ). Inhibition of bacterial quorum sensing by vanilla extract. . Lett Appl Microbiol 42:, 637–641.[PubMed]
    [Google Scholar]
  10. Cox C. D. , Adams P. . ( 1985; ). Siderophore activity of pyoverdin for Pseudomonas aeruginosa . . Infect Immun 48:, 130–138.[PubMed]
    [Google Scholar]
  11. Davies D. G. , Parsek M. R. , Pearson J. P. , Iglewski B. H. , Costerton J. W. , Greenberg E. P. . ( 1998; ). The involvement of cell-to-cell signals in the development of a bacterial biofilm. . Science 280:, 295–298. [CrossRef] [PubMed]
    [Google Scholar]
  12. Dong Y. H. , Wang L. H. , Xu J. L. , Zhang H. B. , Zhang X. F. , Zhang L. H. . ( 2001; ). Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase. . Nature 411:, 813–817. [CrossRef] [PubMed]
    [Google Scholar]
  13. Eberhard A. , Burlingame A. L. , Eberhard C. , Kenyon G. L. , Nealson K. H. , Oppenheimer N. J. . ( 1981; ). Structural identification of autoinducer of Photobacterium fischeri luciferase. . Biochemistry 20:, 2444–2449. [CrossRef] [PubMed]
    [Google Scholar]
  14. Fuqua C. , Greenberg E. P. . ( 2002; ). Listening in on bacteria: acyl-homoserine lactone signalling. . Nat Rev Mol Cell Biol 3:, 685–695. [CrossRef] [PubMed]
    [Google Scholar]
  15. Gao M. , Teplitski M. , Robinson J. B. , Bauer W. D. . ( 2003; ). Production of substances by Medicago truncatula that affect bacterial quorum sensing. . Mol Plant Microbe Interact 16:, 827–834. [CrossRef] [PubMed]
    [Google Scholar]
  16. Givskov M. , de Nys R. , Manefield M. , Gram L. , Maximilien R. , Eberl L. , Molin S. , Steinberg P. D. , Kjelleberg S. . ( 1996; ). Eukaryotic interference with homoserine lactone-mediated prokaryotic signalling. . J Bacteriol 178:, 6618–6622.[PubMed]
    [Google Scholar]
  17. Glessner A. , Smith R. S. , Iglewski B. H. , Robinson J. B. . ( 1999; ). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. . J Bacteriol 181:, 1623–1629.[PubMed]
    [Google Scholar]
  18. Grossman A. D. . ( 1995; ). Genetic networks controlling the initiation of sporulation and the development of genetic competence in Bacillus subtilis . . Annu Rev Genet 29:, 477–508. [CrossRef] [PubMed]
    [Google Scholar]
  19. Hentzer M. , Givskov M. . ( 2003; ). Pharmacological inhibition of quorum sensing for the treatment of chronic bacterial infections. . J Clin Invest 112:, 1300–1307.[PubMed] [CrossRef]
    [Google Scholar]
  20. Hentzer M. , Riedel K. , Rasmussen T. B. , Heydorn A. , Andersen J. B. , Parsek M. R. , Rice S. A. , Eberl L. , Molin S. . & other authors ( 2002; ). Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. . Microbiology 148:, 87–102.[PubMed]
    [Google Scholar]
  21. Huber B. , Eberl L. , Feucht W. , Polster J. . ( 2003; ). Influence of polyphenols on bacterial biofilm formation and quorum-sensing. . Z Naturforsch C 58:, 879–884.[PubMed] [CrossRef]
    [Google Scholar]
  22. Keshavan N. D. , Chowdhary P. K. , Haines D. C. , González J. E. . ( 2005; ). l-Canavanine made by Medicago sativa interferes with quorum sensing in Sinorhizobium meliloti . . J Bacteriol 187:, 8427–8436. [CrossRef] [PubMed]
    [Google Scholar]
  23. Kessler E. . ( 1995; ). β-Lytic endopeptidases. . Methods Enzymol 248:, 740–756. [CrossRef] [PubMed]
    [Google Scholar]
  24. Kessler E. , Safrin M. , Gustin J. K. , Ohman D. E. . ( 1998; ). Elastase and the LasA protease of Pseudomonas aeruginosa are secreted with their propeptides. . J Biol Chem 273:, 30225–30231. [CrossRef] [PubMed]
    [Google Scholar]
  25. Kjelleberg S. , Molin S. . ( 2002; ). Is there a role for quorum sensing signals in bacterial biofilms?. Curr Opin Microbiol 5:, 254–258. [CrossRef] [PubMed]
    [Google Scholar]
  26. Kong K. F. , Jayawardena S. R. , Indulkar S. D. , Del Puerto A. , Koh C. L. , Høiby N. , Mathee K. . ( 2005; ). Pseudomonas aeruginosa AmpR is a global transcriptional factor that regulates expression of AmpC and PoxB β-lactamases, proteases, quorum sensing, and other virulence factors. . Antimicrob Agents Chemother 49:, 4567–4575. [CrossRef] [PubMed]
    [Google Scholar]
  27. Lutter E. , Lewenza S. , Dennis J. J. , Visser M. B. , Sokol P. A. . ( 2001; ). Distribution of quorum-sensing genes in the Burkholderia cepacia complex. . Infect Immun 69:, 4661–4666. [CrossRef] [PubMed]
    [Google Scholar]
  28. Lyon G. J. , Muir T. W. . ( 2003; ). Chemical signaling among bacteria and its inhibition. . Chem Biol 10:, 1007–1021. [CrossRef] [PubMed]
    [Google Scholar]
  29. Manefield M. , Rasmussen T. B. , Henzter M. , Andersen J. B. , Steinberg P. , Kjelleberg S. , Givskov M. . ( 2002; ). Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover. . Microbiology 148:, 1119–1127.[PubMed]
    [Google Scholar]
  30. Mathee K. , McPherson C. J. , Ohman D. E. . ( 1997; ). Posttranslational control of the algT (algU)-encoded σ22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN). . J Bacteriol 179:, 3711–3720.[PubMed]
    [Google Scholar]
  31. Meyer J. M. , Neely A. , Stintzi A. , Georges C. , Holder I. A. . ( 1996; ). Pyoverdin is essential for virulence of Pseudomonas aeruginosa . . Infect Immun 64:, 518–523.[PubMed]
    [Google Scholar]
  32. Mittal R. , Sharma S. , Chhibber S. , Harjai K. . ( 2008; ). Iron dictates the virulence of Pseudomonas aeruginosa in urinary tract infections. . J Biomed Sci 15:, 731–741. [CrossRef] [PubMed]
    [Google Scholar]
  33. O’Toole G. A. , Kolter R. . ( 1998; ). Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. . Mol Microbiol 28:, 449–461. [CrossRef] [PubMed]
    [Google Scholar]
  34. Ohman D. E. , Cryz S. J. , Iglewski B. H. . ( 1980; ). Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. . J Bacteriol 142:, 836–842.[PubMed]
    [Google Scholar]
  35. Parsek M. R. , Greenberg E. P. . ( 2000; ). Acyl-homoserine lactone quorum sensing in Gram-negative bacteria: a signaling mechanism involved in associations with higher organisms. . Proc Natl Acad Sci U S A 97:, 8789–8793. [CrossRef] [PubMed]
    [Google Scholar]
  36. Rasch M. , Buch C. , Austin B. , Slierendrecht W. J. , Ekmann K. S. , Larsen J. L. , Johansen C. , Riedel K. , Eberl L. . & other authors ( 2004; ). An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). . Syst Appl Microbiol 27:, 350–359. [CrossRef] [PubMed]
    [Google Scholar]
  37. Rasmussen T. B. , Bjarnsholt T. , Skindersoe M. E. , Hentzer M. , Kristoffersen P. , Köte M. , Nielsen J. , Eberl L. , Givskov M. . ( 2005; ). Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. . J Bacteriol 187:, 1799–1814. [CrossRef] [PubMed]
    [Google Scholar]
  38. Ren D. , Sims J. J. , Wood T. K. . ( 2002; ). Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone. . Lett Appl Microbiol 34:, 293–299. [CrossRef] [PubMed]
    [Google Scholar]
  39. Rodelas B. , Lithgow J. K. , Wisniewski-Dye F. , Hardman A. , Wilkinson A. , Economou A. , Williams P. , Downie J. A. . ( 1999; ). Analysis of quorum-sensing-dependent control of rhizosphere-expressed (rhi) genes in Rhizobium leguminosarum bv. viciae . . J Bacteriol 181:, 3816–3823.[PubMed]
    [Google Scholar]
  40. Schuster M. , Greenberg E. P. . ( 2006; ). A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa . . Int J Med Microbiol 296:, 73–81. [CrossRef] [PubMed]
    [Google Scholar]
  41. Shaw P. D. , Ping G. , Daly S. L. , Cha C. , Cronan J. E. Jr , Rinehart K. L. , Farrand S. K. . ( 1997; ). Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. . Proc Natl Acad Sci U S A 94:, 6036–6041. [CrossRef] [PubMed]
    [Google Scholar]
  42. Singh B. N. , Singh B. R. , Singh R. L. , Prakash D. , Dhakarey R. , Upadhyay G. , Singh H. B. . ( 2009a; ). Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera . . Food Chem Toxicol 47:, 1109–1116. [CrossRef] [PubMed]
    [Google Scholar]
  43. Singh B. N. , Singh B. R. , Singh R. L. , Prakash D. , Sarma B. K. , Singh H. B. . ( 2009b; ). Antioxidant and anti-quorum sensing activities of green pod of Acacia nilotica L. . Food Chem Toxicol 47:, 778–786. [CrossRef] [PubMed]
    [Google Scholar]
  44. Sitnikov D. M. , Schineller J. B. , Baldwin T. O. . ( 1995; ). Transcriptional regulation of bioluminescence genes from Vibrio fischeri . . Mol Microbiol 17:, 801–812. [CrossRef] [PubMed]
    [Google Scholar]
  45. Surette M. G. , Miller M. B. , Bassler B. L. . ( 1999; ). Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. . Proc Natl Acad Sci U S A 96:, 1639–1644. [CrossRef] [PubMed]
    [Google Scholar]
  46. Teplitski M. , Robinson J. B. , Bauer W. D. . ( 2000; ). Plants secrete substances that mimic bacterial N-acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. . Mol Plant Microbe Interact 13:, 637–648. [CrossRef] [PubMed]
    [Google Scholar]
  47. Venturi V. . ( 2006; ). Regulation of quorum sensing in Pseudomonas . . FEMS Microbiol Rev 30:, 274–291. [CrossRef] [PubMed]
    [Google Scholar]
  48. Von Bodman S. B. , Bauer W. D. , Coplin D. L. . ( 2003; ). Quorum sensing in plant-pathogenic bacteria. . Annu Rev Phytopathol 41:, 455–482. [CrossRef] [PubMed]
    [Google Scholar]
  49. Whitehead N. A. , Welch M. , Salmond G. P. . ( 2001; ). Silencing the majority. . Nat Biotechnol 19:, 735–736. [CrossRef] [PubMed]
    [Google Scholar]
  50. Zhu H. , Sun S. J. . ( 2008; ). Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. . Curr Microbiol 57:, 418–422. [CrossRef] [PubMed]
    [Google Scholar]
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