1887

Abstract

is an intracellular pathogen which causes tularaemia. There is no licensed vaccine currently available for prophylaxis. The γ-glutamyl transpeptidase (GGT) encoded by the gene has been shown to be important for the intracellular survival of . In this study we have constructed a deletion mutant in the highly virulent . strain SCHU S4. Characterization of the mutant strain confirmed the function of , and confirmed the role of GGT in cysteine acquisition. The mutant strain was highly attenuated both and using murine models of infection. Moreover, we have demonstrated that the attenuated mutant is able to induce protective immunity against an SCHU S4 challenge, and thus may be a candidate for the development of an attenuated vaccine.

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2011-11-01
2019-12-06
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