1887

Abstract

head blight (FHB) is a re-emerging wheat disease that causes extensive damage through direct losses in yield and quality due to the presence of damaged kernels and their associated mycotoxins such as the trichothecene deoxynivalenol (DON). Biological control, including the treatment of crop residues with antagonists, in order to reduce pathogen inoculum of FHB, holds considerable promise. Ten isolates, previously selected for their ability to grow in the presence of DON, were preliminarily investigated as potential antagonists against and mycotoxigenic strains in plate confrontation assays. The three isolates showing antibiosis and mycoparasitism were evaluated for their capacity to inhibit DON production by and on two natural substrates. The expression of some chitinase-encoding genes by the two best resulting strains, during interaction with and , was monitored. All investigated genes from chitinase subgroups A, B and the new subgroup C responded to mycoparasitic conditions and were upregulated before contact and/or when in contact with the host. 6085, the best antagonist, was finally used in a competition test against and on natural substrates, using a qPCR approach to evaluate its effect on the pathogen’s growth and DON production in haulms and rice. This test confirmed the ability of 6085 to antagonize the pathogens on rice. On wheat haulms, an extreme oligotrophic environment, 6085 seemed to develop very poorly and the growth of both the pathogens was unaffected by the presence of the antagonist.

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2012-01-01
2019-10-22
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