1887

Abstract

The genome of the highly pathogenic Nagasaki strain (serovar 5) was sequenced to 99 % completion. A genomic comparison with two other pathogenic serovar 5 strains identified six genes per genome () encoding β-arrel onomeric utotransporters, and being pseudogenes in at least one strain. The remaining encoded proteins were predicted to belong to the subtilisin (BmaA1 and BmaA4) and cysteine (BmaA5 and BmaA6) protease families. Allelic polymorphism was detected in other strains by comparative genomic hybridization using microarrays. Recombination events were observed, some of them leading to gene disruption in one of the three strains, although synteny around genes was conserved. These results suggest that genes are undergoing a process of reductive evolution. To evaluate their use as potential vaccine antigens, the products of the passenger domains of , , and were produced in as recombinant proteins. They were detected by immunoblotting using sera of colostrum-deprived piglets recovering from a sublethal infection with (Nagasaki). The existence of specific antibodies after infection with also demonstrated expression. Using proteomics, only BmaA6 was detected in the -grown Nagasaki strain. Interestingly, the translocator domain was found in the outer membrane, while the passenger domain was located in supernatants. These results indicate that BmaA proteins could be considered as immunogen candidates to improve vaccines. However, their capacity to confer protective immunity needs to be studied further.

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2012-02-01
2019-08-19
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