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Volume 158, Issue 1

Comparative study of gene expression in interactions with tomato plants using high-density oligonucleotide microarrays Free

Abstract

spp. are widely used as biopesticides and biofertilizers to control diseases and to promote positive physiological responses in plants. and assays with CECT 2413 (T34), Gv29-8 (T87) and IMI 224801 (T7) revealed that these strains affected the growth and development of lateral roots in tomato plants in different ways. The early expression profiles of these strains were studied after 20 h of incubation in the presence of tomato plants, using a high-density oligonucleotide (HDO) microarray, and compared to the profiles in the absence of plants. Out of the total 34 138 probe sets deposited on the microarray, 1077 (3.15 %) showed a significant change of at least 2-fold in expression in the presence of tomato plants. The numbers of probe sets identified in the individual strains were 593 in T34, 336 in T87 and 94 in T7. Carbohydrate metabolism – the chitin degradation enzymes -acetylglucosamine-6-phosphate deacetylase, glucosamine-6-phosphate deaminase and chitinase – was the most significantly overrepresented process commonly observed in the three strains in early interactions with tomato plants. Strains T7 and T34, which had similar positive effects on plant development in biological assays, showed a significantly overrepresented hexokinase activity in interaction with tomato. In addition, genes encoding a 40S ribosomal protein and a P23 tumour protein were altered in both these strains.

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© 2012 SGM
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2012-01-01
2024-04-19
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Comparative study of Trichoderma gene expression in interactions with tomato plants using high-density oligonucleotide microarrays
Microbiology 158, 119 (2012); https://doi.org/10.1099/mic.0.052118-0
/content/journal/micro/10.1099/mic.0.052118-0
/content/journal/micro/10.1099/mic.0.052118-0
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