1887

Abstract

Penicillin production in is controlled by PcVelA and PcLaeA, two components of the regulatory -like complex. Comparative microarray analysis with mutants lacking PcVelA or PcLaeA revealed a set of 62 common genes affected by the loss of both components. A downregulated gene in both knockout strains is , potentially encoding a class V chitinase. Under nutrient-depleted conditions, transcript levels of are strongly upregulated, and the gene product contributes to more than 50 % of extracellular chitinase activity. Functional characterization by generating -disruption strains revealed that PcChiB1 is responsible for cell wall integrity and pellet formation in Further, fluorescence microscopy with a DsRed-labelled chitinase suggests a cell wall association of the protein. An unexpected phenotype occurred when knockout strains were grown on media containing -acetylglucosamine as the sole C and N source, where, in contrast to the recipient, a penicillin producer strain, the mutants and an ancestral strain show distinct mycelial growth. We discuss the relevance of this class V chitinase for morphology in an industrially important fungus.

Funding
This study was supported by the:
  • Sandoz GmbH
  • Christian Doppler Society
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2011-11-01
2021-04-15
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