1887

Abstract

The human pathogen harbours many genes encoding phosphotransferase systems and sugar ABC (ATP-binding cassette) transporters, including systems for the utilization of the β-glucoside sugar cellobiose. In this study, we show that the transcriptional regulator CelR, which has previously been found to be important for pneumococcal virulence, activates the expression of the cellobiose-utilization gene cluster ( locus) of . Expression directed by the two promoters present in the locus was increased in the presence of cellobiose as sole carbon source in the medium, while expression decreased in the presence of glucose in the medium. Furthermore, we have predicted a 22 bp putative CelR regulatory site (5′-YTTTCCWTAWCAWTWAGGAAAA-3′) in the promoters of and , and analysis showed that it is highly conserved in other pathogenic streptococci as well. Promoter truncations of and , where the half or full CelR regulatory site was deleted, confirmed that the CelR-binding site in P and P is functional. Transcriptome studies with the mutant and in prediction of the CelR regulatory site in the entire D39 genome sequence show that the locus is the only cluster of genes under the direct control of CelR. Therefore, CelR is a regulator dedicated to the cellobiose-dependent transcriptional activation of the locus.

Funding
This study was supported by the:
  • Higher Education Commission (HEC) Pakistan
  • EU (Award HEALTH-F3-2009-222983)
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2011-10-01
2021-08-04
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