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Volume 157, Issue 12

-Acetylglucosamine-dependent biofilm formation in is cryptic and activated by elevated c-di-GMP levels Free

Abstract

The phytopathogenic bacterium () strain SCRI1043 does not exhibit appreciable biofilm formation under standard laboratory conditions. Here we show that a biofilm-forming phenotype in this strain could be activated from a cryptic state by increasing intracellular levels of c-di-GMP, through overexpression of a constitutively active diguanylate cyclase (PleD*) from . Randomly obtained transposon mutants defective in the operon, involved in synthesis and translocation of poly-β-1,6--acetyl--glucosamine (PGA), were all impaired in this biofilm formation. The presence of the PGA-degrading enzyme dispersin B in the growth media prevented biofilm formation by overexpressing PleD*, further supporting the importance of PGA for biofilm formation by . Importantly, a mutant exhibited a reduction in root binding to the host plant under conditions of high intracellular c-di-GMP levels. A modest but consistent increase in transcript levels was associated with high intracellular levels of c-di-GMP. Our results indicate tight control of PGA-dependent biofilm formation by c-di-GMP in .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • BBSRC
  • Consejo Superior de Investigaciones Científicas
  • CSIC
© 2011 SGM

Erratum

An erratum has been published for this content:
-Acetylglucosamine-dependent biofilm formation in is cryptic and activated by elevated c-di-GMP levels
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2011-12-01
2023-09-21
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N-Acetylglucosamine-dependent biofilm formation in Pectobacterium atrosepticum is cryptic and activated by elevated c-di-GMP levels
Microbiology 157, 3340 (2011); https://doi.org/10.1099/mic.0.050450-0
/content/journal/micro/10.1099/mic.0.050450-0
/content/journal/micro/10.1099/mic.0.050450-0
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