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Volume 158, Issue 2

Identification of seven pv. genes potentially involved in pathogenesis in rice Free

Abstract

pv. () causes bacterial leaf streak (BLS) in rice, an emerging and destructive disease worldwide. Identification of key virulence factors is a prerequisite for understanding the pathogenesis of . In this study, a Tn-tagged mutant library of strain RS105 was screened on rice, and 27 Tn mutants were identified that were either non-pathogenic or showed reduced virulence in rice. Fourteen of the non-pathogenic mutants were also unable to elicit the hypersensitive response (HR) in tobacco and were designated Pth/HR mutants; 13 mutants showed attenuated virulence and were able to induce an HR (Vir/HR). Sequence analysis of the Tn-tagged genes indicated that the 14 Pth/HR mutants included mutations in , , , , and . The 13 Vir/HR mutants included (a transcriptional activator-like TAL effector), (regulator of pathogenicity factors), (oxidative stress transcriptional regulator), (disulfide isomerase), (glucan biosynthesis glucosyltransferase H), (glucose-1-phosphate thymidylyltransferase), (aminotransferase), (amidophosphoribosyltransferase), (threonine synthase), (tryptophan synthase alpha subunit) and three genes encoding hypothetical proteins (, and ). Collectively, the 27 Tn insertions are located in 21 different open reading frames. Bacterial growth and virulence assays demonstrated that , , , , , and are candidate virulence genes involved in pathogenesis. Reduced virulence in 13 mutants was restored to wild-type levels when the cognate gene was introduced . Expression profiles demonstrated that the seven candidate virulence genes were significantly induced , although their roles in pathogenesis remain unclear.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© 2012 SGM
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2012-02-01
2024-04-18
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Identification of seven Xanthomonas oryzae pv. oryzicola genes potentially involved in pathogenesis in rice
Microbiology 158, 505 (2012); https://doi.org/10.1099/mic.0.050419-0
/content/journal/micro/10.1099/mic.0.050419-0
/content/journal/micro/10.1099/mic.0.050419-0
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