1887

Abstract

community-acquired pyogenic liver abscess (PLA) is an emerging infectious disease. The gene (for egulator of ucoid henotype A) has been reported to be associated with PLA in prevalence studies. NTUH-K2044, a K1 PLA isolate, carries three genes: two large-plasmid-carried genes (p- and p-) and one chromosomal gene (c-). In this study, we re-examined the role of in PLA pathogenesis to clarify the relationship of and capsular serotype to virulence. Using isogenic gene deletion strains and complemented strains of NTUH-K2044, we demonstrated that only p- enhanced expression of capsular polysaccharide synthesis () genes and capsule production. Nevertheless, the lethal dose and competitive index indicated that p- does not promote virulence in mice. The prevalence of these three and capsular types in 206 strains was investigated. This revealed a correlation of with six PLA-related capsular types (K1, K2, K5, K54, K57 and KN1). However, the correlation of with K1 strains from the West was less obvious than with the strains from Asia (17/22 vs 39/39,  = 0.0019). Among the three genes, p- was the most prevalent. Due to the strong correlation with PLA-related capsular types, p- could serve as a surrogate marker for PLA. We found an association of p- with three widely spaced loci in a large plasmid (30/32). Therefore, could be co-inherited together with virulence genes carried by this plasmid.

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2011-12-01
2020-01-25
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