1887

Abstract

We sought to develop (BI) as a vehicle for the expression of heterologous antigens. Two proteins of enterotoxigenic (ETEC) were expressed in BI: CfaB, a major fimbrial subunit protein, and LTB, the B subunit of heat-labile enterotoxin. The expression of CfaB and LTB in BI was verified by electrophoretic analysis. Sprague–Dawley rats were then subjected to intragastric immunization with BI-CfaB and BI-LTB systems both separately and together. ELISA was used to characterize the serum and mucosal immune responses against ETEC antigens. The immunized rats were intraperitoneally challenged with wild-type ETEC H10407 to study the immune response . The serum titres of IgG and faecal IgA antibodies in the BI-CfaB plus BI-LTB mixed vaccination group were significantly greater than those in the other two groups, which were immunized with a single vaccine (<0.05). However, no significant difference was seen between the two groups that received a single immunization. These results suggest that expressing CfaB and LTB in BI provides a probiotic system with immunogenic properties. Furthermore, the expression of LTB in BI preserved its mucosal adjuvant effect. So this study confirms that BI can be used as a novel oral vaccine expression system for a heterologous antigen and BI-LTB can provide mucosal adjuvant properties.

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2012-02-01
2020-07-11
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References

  1. Adachi J. A., Jiang Z., Cox M., Wood L., DuPont H. L., Mathewson J. J.. ( 2000;). Serum antibody response induced in mice after oral administration of three different antigens of enterotoxigenic Escherichia coli in enteric coated microparticles. J Travel Med7:304–308 [CrossRef][PubMed]
    [Google Scholar]
  2. Barry E. M., Altboum Z., Losonsky G., Levine M. M.. ( 2003;). Immune responses elicited against multiple enterotoxigenic Escherichia coli fimbriae and mutant LT expressed in attenuated Shigella vaccine strains. Vaccine21:333–340 [CrossRef][PubMed]
    [Google Scholar]
  3. Bermúdez-Humarán L. G.. ( 2009;). Lactococcus lactis as a live vector for mucosal delivery of therapeutic proteins. Hum Vaccin5:264–267 [CrossRef][PubMed]
    [Google Scholar]
  4. Bhaskaran S., Jay C. M., Berghman L. R., Wagner G. G., Waghela S. D.. ( 2005;). A single-chain fragment variable recombinant antibody against F5 fimbria of enterotoxigenic Escherichia coli inhibits agglutination of horse red blood cells induced by F5 protein. Vet Res Commun29:463–476 [CrossRef][PubMed]
    [Google Scholar]
  5. Boedeker E. C.. ( 2005;). Vaccines for enterotoxigenic Escherichia coli: current status. Curr Opin Gastroenterol21:15–19[PubMed]
    [Google Scholar]
  6. Bühler T., Hoschützky H., Jann K.. ( 1991;). Analysis of colonization factor antigen I, an adhesin of enterotoxigenic Escherichia coli O78 : H11: fimbrial morphology and location of the receptor-binding site. Infect Immun59:3876–3882[PubMed]
    [Google Scholar]
  7. Byrd W., Cassels F. J.. ( 2006;). The encapsulation of enterotoxigenic Escherichia coli colonization factor CS3 in biodegradable microspheres enhances the murine antibody response following intranasal administration. Microbiology152:779–786 [CrossRef][PubMed]
    [Google Scholar]
  8. Daley A., Randall R., Darsley M., Choudhry N., Thomas N., Sanderson I. R., Croft N. M., Kelly P.. ( 2007;). Genetically modified enterotoxigenic Escherichia coli vaccines induce mucosal immune responses without inflammation. Gut56:1550–1556 [CrossRef][PubMed]
    [Google Scholar]
  9. DuPont H. L.. ( 2008;). Systematic review: prevention of travellers’ diarrhoea. Aliment Pharmacol Ther27:741–751 [CrossRef][PubMed]
    [Google Scholar]
  10. Gaastra W., Svennerholm A. M.. ( 1996;). Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol4:444–452 [CrossRef][PubMed]
    [Google Scholar]
  11. Girard M. P., Steele D., Chaignat C. L., Kieny M. P.. ( 2006;). A review of vaccine research and development: human enteric infections. Vaccine24:2732–2750 [CrossRef][PubMed]
    [Google Scholar]
  12. Guarner F., Malagelada J. R.. ( 2003;). Gut flora in health and disease. Lancet361:512–519 [CrossRef][PubMed]
    [Google Scholar]
  13. Hanniffy S. B., Carter A. T., Hitchin E., Wells J. M.. ( 2007;). Mucosal delivery of a pneumococcal vaccine using Lactococcus lactis affords protection against respiratory infection. J Infect Dis195:185–193 [CrossRef][PubMed]
    [Google Scholar]
  14. Johnson A. M., Kaushik R. S., Francis D. H., Fleckenstein J. M., Hardwidge P. R.. ( 2009;). Heat-labile enterotoxin promotes Escherichia coli adherence to intestinal epithelial cells. J Bacteriol191:178–186 [CrossRef][PubMed]
    [Google Scholar]
  15. Lee J. C., Park J. S., Shepherd S. E., Carey V., Fattom A.. ( 1997;). Protective efficacy of antibodies to the Staphylococcus aureus type 5 capsular polysaccharide in a modified model of endocarditis in rats. Infect Immun65:4146–4151[PubMed]
    [Google Scholar]
  16. Levine M. M., Nalin D. R., Hoover D. L., Bergquist E. J., Hornick R. B., Young C. R.. ( 1979;). Immunity to enterotoxigenic Escherichia coli. Infect Immun23:729–736[PubMed]
    [Google Scholar]
  17. Liu J. K., Hou X. L., Wei C. H., Yu L. Y., He X. J., Wang G. H., Lee J. S., Kim C. J.. ( 2009;). Induction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic Escherichia coli F41 fimbrial protein. Appl Environ Microbiol75:4491–4497 [CrossRef][PubMed]
    [Google Scholar]
  18. López-Gigosos R., García-Fortea P., Reina-Doña E., Plaza-Martín E.. ( 2007;). Effectiveness in prevention of travellers’ diarrhoea by an oral cholera vaccine WC/rBS. Travel Med Infect Dis5:380–384 [CrossRef][PubMed]
    [Google Scholar]
  19. Lund B. M., Baird-Parker T. C., Gould G. W.. ( 2000;). Microbiological Safety and Quality of Foodvol. 2 Gaithersburg, MD: Aspen Publishers;
    [Google Scholar]
  20. Medina M., Vintiñi E., Villena J., Raya R., Alvarez S.. ( 2010;). Lactococcus lactis as an adjuvant and delivery vehicle of antigens against pneumococcal respiratory infections. Bioeng Bugs1:313–325 [CrossRef][PubMed]
    [Google Scholar]
  21. O’Sullivan D. J., Klaenhammer T. R.. ( 1993;). Rapid mini-prep isolation of high-quality plasmid DNA from lactococcus and Lactobacillus spp.. Appl Environ Microbiol59:2730–2733[PubMed]
    [Google Scholar]
  22. Pascual D. W., Hone D. M., Hall S., van Ginkel F. W., Yamamoto M., Walters N., Fujihashi K., Powell R. J., Wu S.. & other authors ( 1999;). Expression of recombinant enterotoxigenic Escherichia coli colonization factor antigen I by Salmonella typhimurium elicits a biphasic T helper cell response. Infect Immun67:6249–6256[PubMed]
    [Google Scholar]
  23. Pizza M., Giuliani M. M., Fontana M. R., Monaci E., Douce G., Dougan G., Mills K. H., Rappuoli R., Del Giudice G.. ( 2001;). Mucosal vaccines: non toxic derivatives of LT and CT as mucosal adjuvants. Vaccine19:2534–2541 [CrossRef][PubMed]
    [Google Scholar]
  24. Sack R. B.. ( 1975;). Human diarrheal disease caused by enterotoxigenic Escherichia coli. Annu Rev Microbiol29:333–353 [CrossRef][PubMed]
    [Google Scholar]
  25. Steinsland H., Valentiner-Branth P., Perch M., Dias F., Fischer T. K., Aaby P., Mølbak K., Sommerfelt H.. ( 2002;). Enterotoxigenic Escherichia coli infections and diarrhea in a cohort of young children in Guinea-Bissau. J Infect Dis186:1740–1747 [CrossRef][PubMed]
    [Google Scholar]
  26. Uesaka Y., Otsuka Y., Lin Z., Yamasaki S., Yamaoka J., Kurazono H., Takeda Y.. ( 1994;). Simple method of purification of Escherichia coli heat-labile enterotoxin and cholera toxin using immobilized galactose. Microb Pathog16:71–76 [CrossRef][PubMed]
    [Google Scholar]
  27. Wang L., Huang J. A., Nagesha H. S., Smith S. C., Phelps A., Holmes I., Martyn J. C., Coloe P. J., Reeves P. R.. ( 1999;). Bacterial expression of the major antigenic regions of porcine rotavirus VP7 induces a neutralizing immune response in mice. Vaccine17:2636–2645 [CrossRef][PubMed]
    [Google Scholar]
  28. Wei C. H., Liu J. K., Hou X. L., Yu L. Y., Lee J. S., Kim C. J.. ( 2010;). Immunogenicity and protective efficacy of orally or intranasally administered recombinant Lactobacillus casei expressing ETEC K99. Vaccine28:4113–4118 [CrossRef][PubMed]
    [Google Scholar]
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