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Volume 157, Issue 11

Lmo0036, an ornithine and putrescine carbamoyltransferase in , participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance Free

Abstract

is a foodborne pathogen causing listeriosis. Acid is one of the stresses that foodborne pathogens encounter most frequently. The ability to survive and proliferate in acidic environments is a prerequisite for infection. However, there is limited knowledge about the molecular basis of adaptation of to acid. Arginine deiminase (ADI) and agmatine deiminase (AgDI) systems are implicated in bacterial tolerance to acidic environments. Homologues of ADI and AgDI systems have been found in lineages I and II strains. Sequence analysis indicated that encodes a putative carbamoyltransferase containing conserved motifs and residues important for substrate binding. Lmo0036 acted as an ornithine carbamoyltransferase and putrescine carbamoyltransferase, representing the first example, to our knowledge, that catalyses reversible ornithine and putrescine carbamoyltransfer reactions. Catabolic ornithine and putrescine carbamoyltransfer reactions constitute the second step of ADI and AgDI pathways. However, the equilibrium of carbamoyltransfer reactions was overwhelmingly towards the anabolic direction, suggesting that catabolic carbamoyltransferase was probably the limiting step of the pathways. was induced at the transcriptional level when was subjected to low-pH stress. Its expression product in exhibited higher catabolic carbamoyltransfer activities under acidic conditions. Consistently, absence of this enzyme impaired the growth of under mild acidic conditions (pH 4.8) and reduced its survival in synthetic human gastric fluid (pH 2.5), and corresponded to a loss in ammonia production, indicating that Lmo0036 was responsible for acid tolerance at both sublethal and lethal pH levels. Furthermore, Lmo0036 played a possible role in virulence.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundations of China (Award 30870068 and 31101829)
  • National Science & Technology (Award 2009BADB9B09)
  • China Postdoctoral Science Foundation (Award 20100481428)
© 2011 SGM
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2011-11-01
2024-04-25
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Lmo0036, an ornithine and putrescine carbamoyltransferase in Listeria monocytogenes, participates in arginine deiminase and agmatine deiminase pathways and mediates acid tolerance
Microbiology 157, 3150 (2011); https://doi.org/10.1099/mic.0.049619-0
/content/journal/micro/10.1099/mic.0.049619-0
/content/journal/micro/10.1099/mic.0.049619-0
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